Figure 2

Microarray analysis of MHV-infected LR7 cells. (A) Genomic viral RNA (vRNA) levels in MHV-infected LR7 cells (MOI 10) were measured by quantitative RT-PCR at the indicated time points. The data are presented as relative vRNA levels. (B) Successful amplification of the mRNA within the individual samples was monitored by analyzing the cRNA samples with a Bioanalyzer (Agilent), according to the manufacturer's instructions. Representative mRNA amplification plots of total RNA samples obtained from mock- or MHV-infected cells at 4 h and 6 h p.i. are shown. The indicated plots represent the size distribution of the total mRNA content present in the samples. The marker peak is indicated by the arrow. Note that the scaling is different between the plots in order to visualize the complete profile. (C) Total RNA was isolated and processed for microarray analysis as described in the Methods section. The scatter plots display the average expression values from independent dye-swap hybridizations (n = 6) for each gene present on the arrays at the indicated time-points p.i. Red spots represent upregulated gene transcripts while green spots represent downregulated gene transcripts upon infection of cells with MHV. The dashed lines indicate the 2-fold change cut-off.