Table 2 PCR primers used for isolation of zebrafish chemokine cDNAs
Chemokine | Forward primer | Reverse primer |
|---|---|---|
CCL-chr25a | 5'-CATCTGCAAGAGATCACAAATC | 5'-GGACAGTTGCATTAAGCAGAAA |
CCL-chr25o | 5'-CATCTCCTGATTCCTTCTGTGA | 5'-GGTCAAACATATGATGAAGCACA |
CCL-chr25s | 5'-CAGCAGAGGAGCTGTGAATG | 5'-AGAATACAAAATTGCCTTTTCCT |
CCL-chr25t | 5'-TCCTGATTCCTTCTGTGATTGA | 5'-CACATCAGAAATAGTACAAAAGGTCA |
CCL-chr25v | 5'-CATCTCCTGATTCCTTCTGTGA | 5'-GGTCAAACATATGATGAAGCACA |
CCL-chr25y | 5'-CACACATTAGATCCAGCTTGAGA | 5'-GGTTCAGAAGTTAGAGTACGCAGA |
CCL-chr25ab | 5'-CACACATTAGATCCAGCTTGAGA | 5'-GGTTCAGAAGTTAGAGTACGCAGA |
CCL-chr25ac | 5'-CACACATTAGATCCAGCTTGAGA | 5'-GGTTCAGAAGTTAGAGTACGCAGA |
CCL-chr24a | 5'-CACTTTCAAGATGGGCAACA | 5'-GAGTTTTAGCCTTAAGATAAAGTGGTG |
CXL-chr24a | 5'-TGTGAAATCCTTCAGCAAACA | 5'-TTCACAGATGTCCAGTTTCCA |
CCL-chr24b | 5'-TCATTCAAGAAATCATTCAGCAA | 5'-TGAAAATTTCTTTCCGGTGTG |
CCL-chr24c | 5'-TCTGTGAAATCATTCAGCAATC | 5'-GAAGTTCTCCGATGTCCAGTC |
CXCL-chr24e | 5'-TGGACGACAAACAGACTCACA | 5'-CAGCATATTTTATAGCATAATGTTCAA |
CCL-chr24j | 5'-CAGCTGACCATCACCAAACA | 5'-AGTTTTGCAGCATCTTCAAGG |
CCL-chr24m | 5'-TCAGATACCAGAAGTGAAACTGTGA | 5'-TGGTGGTATCGTGGAAGTCA |
CCL-chr20d | 5'-GATCAGCCACCGATAAACCT | 5'-ACCAGCAGAGCGTTCTCATC |
CCL-chr20g | 5'-GCATCAAGATCTGCTCATCG | 5'-TGAGAAACGATTCAGTGCAA |
CCL-chr17a | 5'-CAAAACATGATGAGTATTTGTTGGA | 5'-GGAATCATCACTGTCAACTTGC |
CXCL-chr13c | 5'-AGCATCTTCACCTCTTGTATGC | 5'-TTTTGTTGTGCCGAATCTCA |
CXCL-chr13d | 5'-TCTTCAGCCATCAAGAACGTC | 5'-TCTCCTGCAGTGAAACGATG |
CCL-chr11a | 5'-TCTTCCCAGAACCTCTGAGC | 5'-GTTTGTGCATTAAATCAAACGA |
CCL-chr11b | 5'-GACAGCACGGAGGAAAAATC | 5'-TGACACACAAGAAGGCTGGA |
CCL-chr10b | 5'-GAAATCACCCCGATCTGCT | 5'-AGGATCAACACGAGCAAACA |
CCL-chr5a | 5'-CGATAGAGCGGTGAGAGGAG | 5'-TTAGCATAAACAGCTGCCATT |
CXCL-chr5c | 5'-GTTTCATGCAGGGATTTTCC | 5'-TTGTGACATCACGCGGAGGA |
CCL-chr2e | 5'-TGAGCATCTCTACATCTTTGCAT | 5'-AGAGGTATGGAGTCAGTGCTGT |
CXCL-chr1c | 5'-TCAGCAGCACTTCATTCAC | 5'-CAGTGTACAGCGAGCGAATC |
CCL-chrUa | 5'-CAAAACATGATGAGTATTTGTTGGA | 5'-GGAATCATCACTGTCAACTTGC |
CCL-chrUb | 5'-CAAAACATGATGAGTATTTGTTGGA | 5'-GGAATCATCACTGTCAACTTGC |
CCL-chrUc | 5'-CAACTGGTTCATCAAGCATACC | 5'-GGGGTATGTATCCAAGTAAGTGC |
CCL-chrUd | 5'-CATCTCCTGATTCCTTCTGTGA | 5'-GGTCAAACATATGATGAAGCACA |
CCL-chrUe | 5'-CATCAACCACACCAGCCTTA | 5'-AAAGGACAGTTGCATTAAGCAGT |
CCL-chrUi | 5'-TCTTGAGAAGCAGAATCAGCAG | 5'-CAAAGATCATCACCACATCTGAA |
CCL-chrUj | 5'-CAGCAGAGGAGCTGTGAATG | 5'-AGAATACAAAATTGCCTTTTCCT |