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Table 3 Primers used for RT-PCR

From: Extensive expansion and diversification of the chemokine gene family in zebrafish: Identification of a novel chemokine subfamily CX

Chemokine Forward primer Reverse primer Product size (bp)
CCL-chr25s 5'-TTCTGCTGTTTTTGGTGTTCA 5'-GCTTTATCCAGTCCTCATCTGG 216
CCL-chr24a 5'-TACTTCTCAGCTTCCTTGTGGAGA 5'-CTCATCGTCTTCGTCTTCATTGTT 250
CXL-chr24a 5'-AGGTTGGAGCAGTACCGACAA 5'-CCTCCCCTCTACACGTTACCC 245
CCL-chr24c 5'-ATGCGTCTGTCCTCAGCTTT 5'-GCTGTCGGATCTATGCAGTG 284
CCL-chr24m 5'-AGGAGTTTGGCTGTTGCACT 5'-CCTCCTCACCCACAGTTGTC 219
CCL-chr20d 5'-CAGACAAGTGCTGCTTTTCG 5'-GAGCTGGCCTCTGTGTCTTT 209
CCL-chr20g 5'-CGCTTTCTGCTCAGTTGTTG 5'-TCAGTCTCCGAACCCATTTC 215
scyba a 5'-TGCAGATGCACAAGAAAAGG 5'-GGCTTCAAACGTCCTGTGTT 225
cxcl12a a 5'-TTCATGCACCGATTTCCAAC 5'-TGTTGATGGCGTTCTTCAGG 222
CXCL-chr13a 5'-CAACATGTCCCAAAGACCTG 5'-TTACCTGTTCCAACATTTCATTAGA 201
CXCL-chr13c 5'-ACTGCCCATCCAGCAGTTAC 5'-TCCTGCCTTCGATGATCTTT 200
CXCL-chr13d 5'-TTGTTTTTGGAGTCAGCATCA 5'-AGTGTGTTCTGGGGTTCAGG 222
CCL-chr11a 5'-CCAGCATCGCACAAGGTTAC 5'-GCCTGGACCCAGTCACTTCT 208
CCL-chr11b 5'-ATGCAGCGAGATGTTCAGTG 5'-GGAATTCACAACAGCGACCT 282
CCL-chr10a 5'-CGATGATGCAGTGGATTGTT 5'-TTTCCTGGGTCGTTTTTGTG 235
CCL-chr10b 5'-TACTGTGCATCGCCTTCAGC 5'-TAGACGAGCCATTCGCTTCA 233
ccl1 a 5'-GGCACGAGATGGAGTTCAGA 5'-GCCGTCTGTATCCGTGATTT 329
CCL-chr5a 5'-ACGGTCAGCCCAAGAGTCAT 5'-GAGCATGACAGCGTCTCTCG 229
CCL-chr5b 5-CCATAACTCTCCTGCTCATCG 5'-GCGCAGAGTTTCTTGTCCTT 202
CXCL-chr5c 5'-AAGTGCGTGCCTGATAAACC 5'-CACATCAACTGCCTGAATGG 229
CXCL-chr5d 5'-TGTTACTGCTCTGCTTCTTGTCA 5'-CATCGTTTTCCAGCTCCATT 204
CXCL-chr1c 5'-TACGTCCAGCAGGGAGAAGT 5'-CCCTGCTGTTTTTGTGGATT 218
CCL-chrUi 5'-GACTTCTGGCCGTCACATTC 5'-AGCTGAGGGTCCACACAGAG 202
β-actin 5'-TGTTCGAGACCTTCAACACC 5'-TGAAGGTGGTCTCGTGGATA 471
  1. a Bold letters indicate approved gene symbols.