Table 3 Primers used for RT-PCR
Chemokine | Forward primer | Reverse primer | Product size (bp) |
|---|---|---|---|
CCL-chr25s | 5'-TTCTGCTGTTTTTGGTGTTCA | 5'-GCTTTATCCAGTCCTCATCTGG | 216 |
CCL-chr24a | 5'-TACTTCTCAGCTTCCTTGTGGAGA | 5'-CTCATCGTCTTCGTCTTCATTGTT | 250 |
CXL-chr24a | 5'-AGGTTGGAGCAGTACCGACAA | 5'-CCTCCCCTCTACACGTTACCC | 245 |
CCL-chr24c | 5'-ATGCGTCTGTCCTCAGCTTT | 5'-GCTGTCGGATCTATGCAGTG | 284 |
CCL-chr24m | 5'-AGGAGTTTGGCTGTTGCACT | 5'-CCTCCTCACCCACAGTTGTC | 219 |
CCL-chr20d | 5'-CAGACAAGTGCTGCTTTTCG | 5'-GAGCTGGCCTCTGTGTCTTT | 209 |
CCL-chr20g | 5'-CGCTTTCTGCTCAGTTGTTG | 5'-TCAGTCTCCGAACCCATTTC | 215 |
scyba a | 5'-TGCAGATGCACAAGAAAAGG | 5'-GGCTTCAAACGTCCTGTGTT | 225 |
cxcl12a a | 5'-TTCATGCACCGATTTCCAAC | 5'-TGTTGATGGCGTTCTTCAGG | 222 |
CXCL-chr13a | 5'-CAACATGTCCCAAAGACCTG | 5'-TTACCTGTTCCAACATTTCATTAGA | 201 |
CXCL-chr13c | 5'-ACTGCCCATCCAGCAGTTAC | 5'-TCCTGCCTTCGATGATCTTT | 200 |
CXCL-chr13d | 5'-TTGTTTTTGGAGTCAGCATCA | 5'-AGTGTGTTCTGGGGTTCAGG | 222 |
CCL-chr11a | 5'-CCAGCATCGCACAAGGTTAC | 5'-GCCTGGACCCAGTCACTTCT | 208 |
CCL-chr11b | 5'-ATGCAGCGAGATGTTCAGTG | 5'-GGAATTCACAACAGCGACCT | 282 |
CCL-chr10a | 5'-CGATGATGCAGTGGATTGTT | 5'-TTTCCTGGGTCGTTTTTGTG | 235 |
CCL-chr10b | 5'-TACTGTGCATCGCCTTCAGC | 5'-TAGACGAGCCATTCGCTTCA | 233 |
ccl1 a | 5'-GGCACGAGATGGAGTTCAGA | 5'-GCCGTCTGTATCCGTGATTT | 329 |
CCL-chr5a | 5'-ACGGTCAGCCCAAGAGTCAT | 5'-GAGCATGACAGCGTCTCTCG | 229 |
CCL-chr5b | 5-CCATAACTCTCCTGCTCATCG | 5'-GCGCAGAGTTTCTTGTCCTT | 202 |
CXCL-chr5c | 5'-AAGTGCGTGCCTGATAAACC | 5'-CACATCAACTGCCTGAATGG | 229 |
CXCL-chr5d | 5'-TGTTACTGCTCTGCTTCTTGTCA | 5'-CATCGTTTTCCAGCTCCATT | 204 |
CXCL-chr1c | 5'-TACGTCCAGCAGGGAGAAGT | 5'-CCCTGCTGTTTTTGTGGATT | 218 |
CCL-chrUi | 5'-GACTTCTGGCCGTCACATTC | 5'-AGCTGAGGGTCCACACAGAG | 202 |
β-actin | 5'-TGTTCGAGACCTTCAACACC | 5'-TGAAGGTGGTCTCGTGGATA | 471 |