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Table 1 Inducible promoter motifs in P. luminescens in presence of insect larvae homogenate

From: Photorhabdus luminescens genes induced upon insect infection

Clone No.

Insert/size

Fluorescence Induction

No. of identical clones

Intergenic region

Identified promoter motif within the DNA-insert and homology to promoter motif of intergenic regions, [-35 region, -spacer-, -10 region]

1

plu1165 (320 bp)

12.7-fold (± 3.0)

1

No

ttattt cgccagattattac tgacaatat (insert)

     

t g attt ccatcattgatcgc t a acaatat ( plu3479 )

     

ttattt ttaaaaaataaaa-tgacaata g ( plu2400 )

     

ttattt gtccgtcgttat--tgacaa c at( plu3567 )

     

ttattt ttggctatttaa--t a acaatat( plu3608 )

2

rpoH (71 bp)

3.2-fold (± 0.3)

1

no

ttgtta aagttgcaac-aactaaag (insert)

     

g tgtta gtgttaataa-aactaaa a ( plu1672 )

     

t a gtta accacggcgaaaactaaag ( sucA )

     

a tgtta atttatggtt-aactaaa a ( plu1864 )

3

plu0097 (385 bp)

2.8-fold (± 0.1)

1

no

tctatt ggggacgggcgtcc-tggtttgat (insert)

     

tc a att tgtcattttggctaatggtttgat ( hutU )

4

plu1880/plu3515-plu3516 (243 bp)

3.5-fold (± 0.2)

1

no

ttaaag agttgaattcagttatttaaat (insert)

     

tt g aag gataacgggta--tatttaaat ( plu4122 )

5

plu1375 (376 bp)

4.3-fold (± 1.2)

1

no

ttgatg tggattacgtggtt--gattatttt (insert)

     

ttgat t agcctgaattacaggggattatttt ( plu1463 )

6

plu4187 (> 1000 bp)

10.1-fold (± 1.8)

1

no

ttgatt cgacgatatt-ttttatatt (insert)

     

tt a att taaatggtt--ttttatatt ( plu1645 )

     

ttgatt agccaaataatttttat c tt ( plu2652 )

     

ttgatt tctttgtgt--t a ttatatt ( plu2790 )

7

fadL (440 bp)

6.4-fold (± 3.3)

1

no

tttact aaacaaaactgcaccgtc--tttttttct (insert)

     

tttact aagcgcgttgtttactcattg ttttttct( spr )

     

tttact ggatgatacacggtgctt--tttttt g ct ( plu4229 )

8

tdh (> 900 bp)

10.3-fold (± 3.9)

2

no

tcgcca gacacccgatcaccaa-ttttaaaac (insert)

     

tcgaca taatcagctaacaattatttt g aaac ( mlc )

     

tc t cca tttcattggaagggaagc tttaaaac ( tccC1)

9

plu0846/ppsA (plu2628) (> 1000 bp)

13.6-fold (± 5.3)

1

no

ttgcag gtgcaggagcaggagaaaccgc--ggtttaaat (insert)

     

ttgca t gttgtaaaatattacaata------ggttta c at ( plu1012 )

     

ttgca t gttgtaaaatattacaata------ggttta c at ( plu1017 )

     

ttgc c g tcgcgatgcatcttgaaatccatagggt a taaat ( ISPlu16A )

10

plu4479/adhC (> 1000 bp)

7.9 (± 3.4)

1

no

ttcact ctgcttaaggtatt-----tgatgtact (insert)

     

tt t act ctcttgcgtccgactatcgtgatgtac a ( plu1579 )

11

plu1949-plu1950 (944 bp)

10.2-fold (± 4.6)

1

yes

tttatg ccggttgcattaattcataaaaat (insert)

     

tttatg ccggttgcattaattcataaaaat ( plu1950 )

12

plu3608-plu3609 (> 1000 bp)

4.5-fold (± 0.3)

1

yes

ttgtta aaaaatcgttatttttggctatttaat (insert)

     

ttgtta aaaaatcgttatttttggctatttaat ( plu3608 )

13

plu2635/plu0847 (101 bp)

13.6-fold (± 5.3)

1

no

ttgcag gtgcaggagcaggagaaaccgcggtttaaat (insert)

14

agaR-agaZ (961 bp)

3.3-fold (± 0.3)

1

yes

ttgaat ctcaaccctatttagtct (insert)

     

ttgaat ctcaaccctatttagtct ( agaZ )

15

plu3688-plu3689 (> 1000 bp)

4.0-fold (± 0.4)

1

yes

ttatca gaccatccggcagggcaaggggaataaa (insert)

     

ttatca gaccatccggcagggcaaggggaataaa ( plu3688 )

     

ttatca acgggctgttggtttaaa-gg a aataaa ( plu2604 )

16

plu0643 (855 bp)

3.7-fold (± 0.8)

1

no

tggcct gcccgaccaccgattggatatatt (insert)

     

t t gcct tgtccctgtcaga--ggat g tatt ( plu1165 )

17

glnD (474 bp)

6.9-fold (± 2.1)

1

no

tcgaac accgttcacgaatatataat (insert)

     

tc c aac caaattgccg-atat t taat ( eutA )

18

walW (127 bp)

3.2-fold (± 0.3)

1

no

ttaatt gaatcgttgagttgtgatact (insert)

     

t c aatt tggtaatacaaca gtgatact ( plu0801 )

     

ttaa a t atgccagtgtagtctgatact ( plu2809 )

  1. The clones were identified by DFI and the sequence upstream the reporter gene was determined by DNA-sequencing. Fluorescence induction factors were determined by three independent measurements, experimental standard deviation is given in the brackets. The promoter motifs were identified with the Softberry BProm analysis tool and homologous promoter motifs were searched within the intergenic regions of the P. luminescens genome. The -10 and the -35 regions are highlighted in bold, and the mismatches compared to the identified promoter motifs are highlighted in italics. The genes downstream of the promoter motifs are marked.
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BMC Genomics

ISSN: 1471-2164

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