Figure 2
From: PPARalpha-mediated effects of dietary lipids on intestinal barrier gene expression
Expression of PPARα along the longitudinal axis of control and WY14643-treated wild-type mice. QPCR was used to determine the relative expression levels of PPARα in sections isolated along the proximal-distal axis of the small intestine of wild-type mice that received the control diet (white, open bars), or were acutely treated (6 hr) with WY14643 (black, closed bars) (n = 4 per group). Small intestines were divided into 10 equal parts; part 1 refers to the most proximal part (duodenum), part 10 refers to the most distal (terminal ileum). Messenger RNA levels were standardized to cyclophilin; part 1 of the non-treated mice was arbitrarily set to 1. Significance of control versus treated wild-type mice was determined per segment using an unpaired student's t-test. * p-value < 0.05. Data are presented as mean ± standard error.