Table 3 Overview of regulated processes
From: PPARalpha-mediated effects of dietary lipids on intestinal barrier gene expression
Gene symbol | Probeset ID | FC WY | FC EPA | FC DHA | FC OA | Function |
|---|---|---|---|---|---|---|
Fatty acid oxidation | ||||||
Fatp4 (Slc27a4) | 1424441_at | 1.7 | 1.5 | 1.4 | 1.4 | Apical long chain FA uptake [76] |
Cact (Slc25a20) | 1423109_s_at | 6.8 | 2.6 | 2.4 | nc | Carnitine cycle in mitochondrial β-oxidation [62] |
Octn2 (Slc22a5) | 1421848_at | 7.7 | 3.2 | 4.2 | nc | Apical carnitine uptake for mitochondrial β-oxidation [48] |
Fatp2 (Slc27a2) | 1416316_at | 2.2 | 1.6 | nc | nc | Has acyl CoA properties to activate FA for subsequent peroxisomal β-oxidation [86] |
Abcd3 | 1416679_at | 2.8 | 1.7 | 1.8 | 1.4 | Transport of VLFA over the peroxisomal membrane for peroxisomal β-oxidation [77] |
Cyp4a10 | 1424853_s_at | 1447.2 | 120.2 | 160.3 | nc | ω-oxidation [87] |
Cyp4b1 | 1416194_at | 1.7 | nc | nc | nc | ω-oxidation [87, 88] |
Cyp4f16 | 1417277_at | 1.9 | 1.5 | 1.4 | 1.9 | ω-oxidation [89, 90] |
G6pt1 (Slc37a4) | 1417042_at | -1.5 | nc | nc | nc | Glycogenolysis [91] |
Nadc1 (Slc13a2) | 1418857_at | -2.1 | -2.2 | nc | nc | Dicarboxylates uptake for Krebs cycle [92] |
Cholesterol flux | ||||||
Npc1l1 | 1438514_at | -1.5 | -1.4 | -1.6 | nc | Apical cholesterol uptake [93] |
Abca1 | 1450392_at | 12.1 | 3.2 | nc | nc | Basolateral cholesterol efflux [56, 58] |
Glucose transport | ||||||
Sglt1 (Slc5a1) | 1455431_at | -1.4 | nc | nc | nc | Apical glucose uptake [94] |
Glut2 (Slc2a2) | 1449067_at | -1.4 | nc | nc | nc | Basolateral glucose efflux [95] |
Glut1 (Slc2a1) | 1426599_a_at | -1.5 | nc | nc | nc | Glucose transport [95] |
Sglt4 (Slc5a9) | 1439494_at | -1.7 | -1.7 | -2.0 | nc | Glucose + mannose transport [96] |
Amino acid metabolism | ||||||
Eaac1 (Slc1a1) | 1448299_at | -1.4 | nc | nc | nc | Apical glutamate uptake [97] |
Pat1 (Slc36a1) | 1428793_at | nc | -1.6 | -1.8 | nc | Apical neutral amino acids uptake [98] |
Lat2 (Slc7a8) | 1417929_at | -1.6 | -1.6 | -2.0 | nc | Basolateral neutral amino acids efflux [99] |
Tat1 (Slc16a10) | 1436368_at | -1.7 | -1.4 | -1.8 | nc | Basolateral aromatic amino acids efflux [100] |
y+Lat1 (Slc7a7) | 1447181_s_at | 1.5 | nc | nc | nc | Basolateral cationic amino acids efflux [99] |
Aralar1 (Slc25a12) | 1428440_at | -1.3 | nc | nc | nc | Malate-aspartate shuttle: provides cytosolic aspartate [62] |
Intestinal motility | ||||||
Sert (Slc6a4) | 1417150_at | -1.4 | -1.2 | -1.3 | nc | Serotonine uptake [66] |
Dat1 (Slc6a3) | 1417415_at | nc | 1.8 | nc | 2.6 | Dopamine uptake [68] |
Nas1 (Slc13a1) | 1430804_at | -2.1 | -2.0 | nc | -2.3 | Apical sulphate uptake [101] |
Oxidative stress | ||||||
Dic (Slc25a10) | 1416954_at | 1.6 | 1.5 | nc | nc | Pyruvate metabolism [62] |
Kmcp1 (Slc25a30) | 1420836_at | 5.5 | nc | nc | nc | ? |
Mct13 (Slc16a13) | 1453056_at | 10.7 | 1.5 | 1.8 | nc | ? |
Svct1 (Slc23a1) | 1421912_at | nc | 1.7 | nc | nc | Apical vitamin C uptake [102] |
Svct2 (Slc23a2) | 1445589_at | 1.4 | nc | nc | nc | Basolateral vitamin C uptake [102] |
Cyp2c29 | 1417651_at | nc | 3.2 | nc | nc | Phase I metabolism |
Cyp2c65 | 1429994_s_at | 2.6 | 2.3 | 2.5 | 1.7 | Phase I metabolism |
Cyp2d22 | 1419039_at | 1.7 | 1.5 | nc | nc | Phase I metabolism |
Akr1b8 | 1448894_at | 13.4 | 3.2 | 5.1 | nc | ? |
Akr1c12 | 1422000_at | -1.5 | nc | nc | nc | Aldo-ketoreductase activity (Phase II) |
Akr1c13 | 1418672_at | -1.7 | nc | nc | nc | Aldo-ketoreductase activity (Phase II) |
Ephx1 | 1422438_at | 1.7 | 1.6 | 1.9 | nc | Epoxide hydrolase activity (Phase II) |
Ephx2 | 1448499_a_at | 1.5 | 1.4 | nc | nc | Epoxide hydrolase activity (Phase II) |
Gsta1///Gsta2 | 1421041_s_at | nc | 1.2 | 1.3 | nc | Glutathione transferase activity (phase II) |
Gsta3 | 1423436_at | nc | nc | 1.8 | nc | Glutathione transferase activity (phase II) |
Gsta4 | 1416368_at | nc | nc | 1.5 | nc | Glutathione transferase activity (phase II) |
Gstk1 | 1452823_at | 1.3 | 1.3 | 1.5 | nc | Glutathione transferase activity (phase II) |
Gstm1 | 1448330_at | nc | 1.7 | nc | nc | Glutathione transferase activity (phase II) |
Gstm3 | 1427473_at | nc | 2.4 | 2.6 | nc | Glutathione transferase activity (phase II) |
Gstm4 | 1424835_at | 2.0 | 1.8 | 1.8 | nc | Glutathione transferase activity (phase II) |
Gstm5 | 1416842_at | -1.3 | nc | nc | nc | Glutathione transferase activity (phase II) |
Gstm6 | 1422072_a_at | nc | 1.3 | nc | nc | Glutathione transferase activity (phase II) |
Gstt2 | 1417883_at | 1.2 | nc | nc | nc | Glutathione transferase activity (phase II) |
Mgst1 | 1415897_a_at | 1.4 | 1.3 | 1.2 | nc | Glutathione transferase activity (phase II) |
Abcg2 | 1422906_at | 1.9 | nc | 1.3 | nc | Apical heme secretion [74] |