Insight into the sialome of the castor bean tick, Ixodes ricinus

Chmelař, Jindřich; Anderson, Jennifer M; Mu, Jianbing; Jochim, Ryan C; Valenzuela, Jesus G; Kopecký, Jan

doi:10.1186/1471-2164-9-233

BMC Genomics

Table 1 Contigs with more than 5 ESTs and their distribution among the four libraries

From: Insight into the sialome of the castor bean tick, Ixodes ricinus

				IRUF			IR24H			IR4D			IR7D
				p	p	p	p
Contig	Comments	Class	EST	obs	exp	IRUF/IR24H	obs	exp	IR24H/IR4D	obs	exp	IR4D/IR7D	obs	exp	ALL
83	cytochrome oxidase 3	h/meten	23	1	5.91	0.004	1	6.02	0.001	12	5.80	0.002	9	5.28	0.000
78	cytochrome oxidase subunit 1	h/meten	22	0	5.65	0.004	2	5.75	0.060	8	5.54	0.001	12	5.05	0.000
88	cytochrome c oxidase subunit II	h/meten	17	6	4.37	0.070	1	4.45	0.101	4	4.28	0.285	6	3.90	0.215
89	ATP synthase F0 subunit 6	h/meten	16	1	4.11	0.062	2	4.19	0.241	5	4.03	0.021	8	3.67	0.031
41	collagen-like secreted protein	s/CLSP	14	0	3.59	0.000	10	3.66	0.000	1	3.53	0.177	3	3.22	0.001
106	elongation factor-1alpha	h/ps	14	3	3.59	0.719	4	3.66	0.184	6	3.53	0.071	1	3.22	0.332
46	collagen-like secreted protein	s/CLSP	12	0	3.08	0.000	11	3.14	0.000	1	3.02	0.043	0	2.76	0.000
87	16S mitochondrial RNA	rRNA	12	3	3.08	0.519	2	3.14	0.192	5	3.02	0.221	2	2.76	0.588
117	Monolaris II group	s/Kunitz	11	0	2.82	0.000	11	2.88	0.000	0	2.77	0.021	0	2.53	0.000
49	collagen-like secreted protein	s/CLSP	11	0	2.82	0.036	5	2.88	0.208	3	2.77	0.743	3	2.53	0.210
116	putative 19 kDa secreted protein	s/19	10	0	2.57	0.000	10	2.62	0.000	0	2.52	0.028	0	2.30	0.000
66	basic tail secreted protein	s/BTSP	10	0	2.57	0.029	5	2.62	0.131	2	2.52	0.570	3	2.30	0.165
56	collagen-like secreted protein	s/CLSP	8	0	2.05	0.042	0	2.09	0.000	8	2.02	0.000	0	1.84	0.000
42	collagen-like secreted protein	s/CLSP	8	0	2.05	0.042	0	2.09	0.148	2	2.02	0.002	6	1.84	0.003
23	collagen-like secreted protein	s/CLSP	7	0	1.80	0.057	0	1.83	0.058	0	1.76	0.000	7	1.61	0.000
48	collagen-like secreted protein	s/CLSP	7	0	1.80	0.000	7	1.83	0.000	0	1.76	0.066	0	1.61	0.000
63	basic tail secreted protein	s/BTSP	7	0	1.80	0.001	6	1.83	0.002	1	1.76	0.164	0	1.61	0.004
68	basic tail secreted protein	s/BTSP	7	0	1.80	0.001	6	1.83	0.002	1	1.76	0.164	0	1.61	0.004
118	cytochrome b	h/meten	7	0	1.80	0.178	2	1.83	0.182	0	1.76	0.003	5	1.61	0.013
85	16S mitochondrial RNA	rRNA	7	1	1.80	0.294	3	1.83	0.204	3	1.76	0.116	0	1.61	0.310
130	60S ribosomal protein L22	h/psrp	7	3	1.80	0.365	2	1.83	0.182	0	1.76	0.173	2	1.61	0.442
14	collagen-like secreted protein	s/CLSP	6	0	1.54	0.078	0	1.57	0.000	6	1.51	0.000	0	1.38	0.000
134	60S ribosomal protein L3	h/psrp	6	6	1.54	0.000	0	1.57	0.079	0	1.51	0.089	0	1.38	0.001
13	collagen-like secreted protein	s/CLSP	6	0	1.54	0.003	5	1.57	0.003	0	1.51	0.204	1	1.38	0.014
5	collagen-like secreted protein	s/CLSP	6	0	1.54	0.078	0	1.57	0.189	2	1.51	0.023	4	1.38	0.041
133	40s ribosomal protein S27	h/psrp	6	4	1.54	0.019	0	1.57	0.189	2	1.51	0.215	0	1.38	0.070
159	5.3 kDa secreted protein	s/4.9	5	0	1.28	0.107	0	1.31	0.109	0	1.26	0.000	5	1.15	0.001
64	basic tail secreted protein	s/BTSP	5	0	1.28	0.001	5	1.31	0.001	0	1.26	0.121	0	1.15	0.003
7	collagen-like secreted protein	s/CLSP	5	0	1.28	0.001	5	1.31	0.001	0	1.26	0.121	0	1.15	0.003
137	NADH dehydrogenase 3	h/meten	5	0	1.28	0.244	1	1.31	0.248	0	1.26	0.004	4	1.15	0.021
24	collagen-like secreted protein	s/CLSP	5	0	1.28	0.244	1	1.31	0.014	4	1.26	0.008	0	1.15	0.037
158	basic tail secreted protein	s/BTSP	5	2	1.28	0.191	0	1.31	0.054	3	1.26	0.059	0	1.15	0.153
50	collagen-like secreted protein	s/CLSP	5	0	1.28	0.199	2	1.31	0.096	3	1.26	0.059	0	1.15	0.157
142	Monolaris II group	s/Kunitz	5	0	1.28	0.199	2	1.31	0.096	3	1.26	0.059	0	1.15	0.157
157	60S ribosomal protein L7A	h/psrp	5	3	1.28	0.058	0	1.31	0.187	2	1.26	0.208	0	1.15	0.158
154	60S ribosomal protein L31	h/psrp	5	3	1.28	0.124	1	1.31	0.248	0	1.26	0.258	1	1.15	0.301
151	40S ribosomal protein S12	h/psrp	5	2	1.28	0.492	1	1.31	0.476	2	1.26	0.208	0	1.15	0.560
131	unknown	uk	5	2	1.28	0.382	2	1.31	0.202	0	1.26	0.258	1	1.15	0.562

P values show significance of expression difference between two libraries; P – ALL shows which contig has random and non-random distribution. Significant values (P ≤ 0.05) are marked in bold. Obs – observed, Exp – expected, h/– housekeeping, s/– secreted, 19 – 19 kDa protein, 4.9 – 4.9 kDa protein, BTSP – basic tail secreted protein, CLSP – collagen-like secreted protein, Kunitz – Kunitz domain containing protein, meten – energy metabolism, ps – proteosynthesis, psrp – proteosynthesis ribosomal protein, rRNA – ribosomal RNA, uk – unknown.

Back to article page

ISSN: 1471-2164

Contact us

Submission enquiries: bmcgenomics@biomedcentral.com
General enquiries: ORSupport@springernature.com