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Table 1 Primer pairs design for each EST.

From: Sequence polymorphism can produce serious artefacts in real-time PCR assays: hard lessons from Pacific oysters

a [GenBank:EX956386]

Primer pairs

Oligonucleotide sequences (5'--3')

Start

Length

Tm

%GC

Amplicon length

f1

CCGACCGTCAAGATTTGAGG

16

20

59

55

51

r1

GTGTAAGGGTCCCTGCCGT

66

19

58

63

 

f2

GCTCTGGATGAAGAAAGGGTGA

116

22

59

50

51

r2

AACGCACCGTTTGACTAAAGC

166

21

58

48

 

f3

ACAAGAATCACGGCAGGGAC

39

20

59

55

51

r3

GACCGGCTGAGAAAGGAACATAT

89

21

59

52

 

f4

ACTGCGTACCAATTCCGACC

2

20

59

55

51

r4

GCCGTGATTCTTGTAGTCCTCAA

52

23

59

48

 

f5

CCAATTCCGACCGTCAAGAT

10

20

58

50

51

r5

GGGTCCCTGCCGTGATTC

60

18

60

67

 

f6

CGTCAAGATTTGAGGACTACAAGAAT

21

26

58

38

51

r6

CATATGTGTAAGGGTCCCTGCC

71

22

59

55

 

f7

ATCACGGCAGGGACCCTTA

45

19

59

58

51

r7

TCCTTGGACCGGCTGAGA

95

18

59

61

 

f8

GGCAGGGACCCTTACACATATG

50

22

59

55

51

r8

ACAATTCCTTGGACCGGCT

100

19

58

53

 

f9

GTCCAAGGAATTGTATCGGTCAA

87

23

59

43

51

r9

TCACCCTTTCTTCATCCAGAGC

137

22

59

50

 

f10

CTCAGCCGGTCCAAGGAAT

79

19

59

58

54

r10

CTTTCTTCATCCAGAGCAAAATTTT

132

25

58

32

 

f11

AATTTTGCTCTGGATGAAGAAAGG

110

24

59

38

51

r11

CCGTTTGACTAAAGCACCAATCA

160

23

60

43

 

f12

TTGCTCTGGATGAAGAAAGGGT

114

22

59

45

51

r12

CGCACCGTTTGACTAAAGCAC

164

21

59

52

 

f13

CGACCGTCAAGATTTGAGGACTA

17

23

59

48

73

r13

GACCGGCTGAGAAAGGAACA

89

20

59

55

 

f14

CCAAGGAATTGTATCGGTCAAAA

89

23

59

39

77

r14

ACGCACCGTTTGACTAAAGCA

165

21

59

48

 

b [GenBank:AJ565694]

Primer pairs

Oligonucleotide sequences (5'--3')

Start

Length

Tm

%GC

Amplicon length

f1

CCGGATATGGGACAAACAAATC

148

22

60

45

67

r1

TGGAAACGATGTCGGCTATG

215

20

60

50

 

f2

CCTTCATGTATGGGTCCCAAA

169

21

58

48

51

r2

ACTCTGGAAACGATGTCGGC

219

20

59

55

 

f3

GTTCCGCAAGGACTATCTCGA

22

21

58

52

51

r3

AAGCTCCCACAGAATTTATTGATGT

72

25

58

36

 

f4

ACTCCAAGGCATGTAGCATCG

313

21

59

52

51

r4

TTGGATGACTGTGCC CTTAAAAT

363

23

59

39

 

f5

TAACATCAATAAATTCTGTGGGAGCT

46

27

59

33

51

r5

AATTTTCGTGCATCTTTCTCTGC

96

23

59

39

 

f6

TGGGACAAACAAATCCTTCATG

155

22

58

41

51

r6

GTCGGCTATGACCTGATTTGG

205

21

58

52

 

f7

CCATGATTCGAACACATTGGTG

241

22

60

45

51

r7

ATGCATGATTCCTTGGCCTTA

291

21

58

43

 

f8

CCAAGGCATGTAGCATCGCT

316

20

60

55

51

r8

TTTTTGGATGACTGTGCCCTTA

366

22

58

41

 

f9

AATTCTGTGGGAGCTTTGCAG

57

22

60

45

51

r9

TCTTTCCTAATAATTTTCGTGCATCTT

107

27

59

30

 

f10

ATGGGTCCCAAATCAGGTCA

178

20

59

50

51

r10

GTGGAAGCAACTCTGGAAACG

228

21

58

52

 

f11

GCAAGAAAACCGGATATGGGA

139

21

60

48

51

r11

TTTGGGACCCATACATGAAGG

189

21

58

48

 

f12

CATGATTCGAACACATTGGTGG

242

22

60

45

51

r12

GATGCATGATTCCTTGGCCT

292

20

59

50

 

f13

TATGGGTCCCAAATCAGGTCA

177

21

59

48

51

r13

TGGAAGCAACTCTGGAAACGAT

227

22

60

45

 

f14

CGTTTCCAGAGTTGCTTCCAC

208

21

58

52

51

r14

AATGTGTTCGAATCATGGTCGTT

258

23

59

39

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