Figure 1

The PriMir pipeline. Prediction and verification of Nmir_018. A. The secondary structure of ml-ncRNA A530020N14 as predicted by RNAfold [44, 45]. B. Among the six hairpins extracted from the ml-ncRNA, four are conserved between mouse and rat (blue frame). The green dashed line indicates the pre-miRNA 5' and 3' end positions predicted by PriMir. Of the four conserved hairpins, one had a PriMir score above 7, and was regarded as a pre-miRNA candidate (pre-Nmir_018; red frame). C. The upper part of the panel shows the predicted pre-Nmir_018, red color letters indicating the position of the mature miRNA Nmir_018. The lower part shows the microarray slide (left) with a positive signal for Nmir_018 (white frame), and an agarose gel electrophoresis (right) of the loop-stem RT-PCR fragment for Nmir_018 (white frame).