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Figure 1 | BMC Genomics

Figure 1

From: Cloning, characterization, and expression of microRNAs from the Asian malaria mosquito, Anopheles stephensi

Figure 1

Northern analysis of eight miRNAs across different developmental stages in An. stephensi. Shown here are eight northern blots performed using Dig-labeled miRCURY LNA probes designed for hybridization to either miR-14, let-7, miR-9a, miR-210, or to one of the four novel miRNAs (miR-x1–x4). The top panels are northern results and the bottom panels are RNA gels for verification of small ribosomal and tRNA integrity and equal loading of total RNA. ssDNA size markers (19 and 23 nts, not shown) were also visualized on the RNA gel for size estimation. Ten micrograms of total RNA for each sample were used. Developmental stages examined were early embryo (Embryo 0–24: 0–24 hrs after egg deposition), late embryo (Embryo 24–41: 24–41 hrs after egg deposition), intermediate and late larval stages (II and IV, respectively), Pupa (P), and Adult (A). To be consistent with our cloning experiment, 17-day old adult females were used in these northern experiments.

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