Lantibiotic prodiction by B. longum DJO10A. (A) Organization of the lantibiotic encoding unique region 12 of B. longum DJO10A and the corresponding genome locations in strains NCC2705 and DJO10A-JH1. The A or B designator following IS30 refer to unique classes of IS30 elements that are only found at this location in the genome. The ' designator indicates a fragmented IS30 element. (B) Pulsed Field Gel Electrophoresis (PFGE) analysis of Xba I-digested total DNA from B. longum DJO10A and its fermentation adapted isolate, DJO10A-JH1. White arrows indicate bands missing from strain DJO10A-JH1. (C) Bioassay for lantibiotic production by B. longum DJO10A with strains DJO10A and DJO10A-JH1 as indicator bacteria.