Figure 1

Selected clusters of phylogenetic tree of the FBG domains in the 12 Drosophila species. The seed sequence used for constructing the tree was the multiple sequence alignment of FBG domains that excluded truncated FBG domains. Bootstrap was applied to the data. Protein distance was calculated using the Jones-Taylor-Thornton model of change between amino acids and a Hidden Markov Model (HMM) method of inferring different rates of evolution at different amino acid positions. Neighbor-joining was applied to produce the tree. The FBG domains of each FREP are denoted by their gene name or GLEANR gene ID. A. A cluster composed of FBG domains from multiple species (Group D in supplement fig. 2). B. A cluster composed of FBG domains from a single species (D. grmshawii, Group K in supplement fig. 2). C. Majority of FBG domains from D. virilis, D. mojavensis and D. grimshawi are clustered together (Group G in supplement fig. 2).