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Figure 2 | BMC Genomics

Figure 2

From: Developing and applying a gene functional association network for anti-angiogenic kinase inhibitor activity assessment in an angiogenesis co-culture model

Figure 2

HUVEC passage dependent angiogenic competence in cord formation assay. HUVECs cultured to various passage numbers (P6, early; P14, intermediate; and P22, late) were co-cultured with NHDFs in the cord formation assay. The cord was visualized by human CD31 staining (green) and quantitated. The cultures were treated with or without 20 ng/ml exogenously added VEGF. Representative images are shown in (A). 6 well-level parameters were quantitated and plotted in (B). Please note that HUVECs gradually lost their cord formation competence when passaged in vitro. Data are expressed as Mean ± S.D. (C-D). HUVECs with various passage numbers (P5, P8, P12, and P22) were assayed for basal gene expression using the Affymetrix GeneChip platform. A linear regression method was used to identify differentially expressed genes over cell passage. An enrichment testing was used to identify significantly changed Biological Process (BP) categories associated with HUVEC passage-dependent gene expression. Representative BP categories were shown using heatmap visualization, for genes associated with mitotic cell cycle (C), and cell death (D).

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BMC Genomics

ISSN: 1471-2164

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