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Figure 5 | BMC Genomics

Figure 5

From: Developing and applying a gene functional association network for anti-angiogenic kinase inhibitor activity assessment in an angiogenesis co-culture model

Figure 5

Analysis of the involvement of MYC oncogene in angiogenesis. (A). Subnetwork analysis of MYC in the interactome. Differentially expressed genes identified from the HUVEC and NHDF microarray studies were used to build a gene regulation interactome as described in the method section. Genes connected to MYC were extracted from the interactome and visualized by building a MYC centric subnetwork. (B). Validation of the involvement of MYC in cord formation by siRNA mediated gene silencing. Expression of MYC in the HUVECs was attenuated by transfecting the cells with siRNA against MYC (siMYC). The siRNA treated HUVECs were co-cultured with regular NHDF cells. siRNA against luciferase (siLUC) was used as the negative control. Knocking down the MYC expression in HUVECs impaired cord formation either in the presence or absence of 20 ng/ml VEGF. (C). Quantitation of cord area and angiogenesis index from the siRNA experiment. (D). Validation of reduced MYC expression in the siMYC treated HUVECs by TaqMan RT-PCR analysis. Data are expressed as Mean ± S.D.

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BMC Genomics

ISSN: 1471-2164

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