Figure 3

Schematic representation of the experimental design for xenobiotic exposure. The figure depicts the 5 doses used for each chemical exposure, together with the number of true (independent) biological replicates, each replicate consisting of 8 (pooled) individual earthworms that have been independently exposed to the relevant treatment condition. Control groups contained 8 replicates, and each of the treatment conditions (i.e. chemical and dose) contained 5 replicates. The design of the array experiment shows the labelling, hybridisation and analysis steps for each biological replication.