Figure 7
Transcriptional responses of adult L. rubellus exposed to a series of concentrations of Atrazine. The array data used for this analysis were normalised per chip and per gene median polishing, expressed relative to the control samples; data from poor quantity spots removed and genes showing significant (p < 0.05) differential expression in response to Atrazine identified using ANOVA analysis with Bonferroni False Discovery. Hierarchical clustering was performed using a distance Algorithm for both conditions and genes. Panel A shows hierarchical clustering of the individual samples, whilst Panel B provides that hierarchical clustering of average expression generated by treatments. Panel C displays the average expression of a series of important functional groups pertinent to Atrazine toxicosis. Full details of the provenance of these reporters are provided in Additional File 12.