Figure 8
Transcript profiles for individual gene responses to xenobiotic exposure. The expression profiles of eight specific reporters are provided to illustrate changes in expression during xenobiotic exposure relative to that observed in control organisms. Transcript changes are shown in response to; Cadmium (exposures 0, 13, 44, 148 and 500 mg/kg) represented by open circles connected by solid lines; Fluoranthene (exposures 0, 14, 47, 158 and 553 mg/kg) designated by open squares and doted line and Atrazine (exposures 0, 9, 20, 35 and 59 mg/kg) represented by open triangles connected by dashed lines. Panels A & B show the profiles of two invariant control genes [73] GAPHD (Lr_Cd2CF_65D0; GenBank accession: DR077591, Cluster:LRC04105_1) and EF1α (Lr_AT1CF_14E04; GenBank: CO047675, Cluster: LRC04881_1). Panel C and D depict the expression profiles for Metallothionein 2B (Lr_Cd2CF_20E01; GenBank: CF611058 Cluster:LRC01607_2) and Laminin (Lr_LC1ED_11A01 GenBank: CF416412 Cluster:LRC01132_1), genes that exhibit compound-specific up- and down-regulation, respectively, in response to cadmium. Panels E & G show reporters Lr_PAHCF_72C10 (GenBank: CO048307, Cluster:LRC05297_1) and Lr_Cu2CF_15B05 (GenBank: DR008743, Cluster:LRC08839_1) specifically induced by Fluoranthene and Atrazine, respectively. Panels F & H show reporters Lr_AT1CF_27G09 (GenBank: CO046812, Cluster:LRC04302_1) and Lr_AT1CF_15E02 (GenBank: CO047812, Cluster:LRC04973_1) exhibiting reciprocal repression by exposure to the PAH and herbicide, respectively.