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Figure 5 | BMC Genomics

Figure 5

From: Genome-wide analysis of antisense transcription with Affymetrix exon array

Figure 5

Validation of antisense transcripts by orientation-specific reverse transcription (RT) PCR. A total of 23 novel antisense transcripts detected by microarray were included. In addition, one known sense-antisense pair (ADCY3 and CENPO), which is marked as "C", was included as a positive control. For each genomic locus, three RT reactions were performed: (a) RT with reverse primer, targeting sense transcript (b) RT with forward primer, targeting antisense transcript, and (c) RT without primer, as negative control. Bolded primer IDs indicate bands that were considered significant in antisense transcription.

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