A simple and accurate SNP scoring strategy based on typeIIS restriction endonuclease cleavage and matrix-assisted laser desorption/ionization mass spectrometry

Table 4 Comparison of RFMP with Snapshot assays for SNP scoring

	Snapshot	RFMP
Assay chemistry	Minisequencing	Restriction endonuclease digestion
Analyte	Primer extended product	Enzyme-digested amplicon
	(single strand-origin)	(both strands-origin)
Assay platfom	Automatic sequencer	MALDI-TOF MS
Accuracy^a	99.5%	99.7%
Throughput/day	2,256^b	18,336
Cost per reaction^c	US$2.50	US$2.00

a, Concordance with Sanger sequencing
b, With a 96-capillary system
c, The estimations include reagents and consumables for PCR and post PCR reactions, and the running cost and amortization of each detection platform except labor charge.

ISSN: 1471-2164