The first generation of a BAC-based physical map of Brassica rapa

BMC Genomics

Table 1 Characteristics of the three source BAC libraries of Brassica rapa ssp. pekinensis cv. Chiifu that were used in the HICF map.

Libraries^a	Genomic DNA partially digested with	Average insert size (kb)	No. of 384 plate	No. of BACs	Average no. of valid bands per clones^b	Genome coverage^c	No. of BACs with successful fingerprints	No. of BACs with repetitive sequences
KBrH	Hin dIII	125	KBrH001-147	56,448	124	12.9×	53,443	16,402
KBrB	Bam HI	126	KBrB001-096	36,864	104	8.5×	34,371	9,604
KBrS	Sau 3AI	100	KBrS001-016	6,144	94	1.2×	5,875	215
Total		124	259	99,456	114	22.5×	93,689	26,221

^aFor details of the BAC libraries, see [7] and [8].
^bValid bands are those in the range of 50~500 bp.
^cGenome coverage was estimated based on the haploid genome equivalent of B. rapa as 550 Mb.

ISSN: 1471-2164