Figure 4

Comparison of the conserved human and chicken patatin domain core modules. The GenBank accession numbers for the chicken and human sequences are provided in Table 1. The identities between full-length patatin domains of the human and chicken PNPLA proteins are for PNPLA1, 56%; PNPLA2, 82%; PNPLA4, 74%; PNPLA6, 88%; PNPLA7, 81%; PNPLA8, 84%; PNPLA9, 74%. The partially identified patatin domain of chicken PNPLA3 is 62% identical with the human ortholog. The numbers in brackets between the active site motifs (in red) represent amino acids that are not shown in the alignment. The numbers behind the sequences show the final positions of the displayed sequences with regard to the full-length amino acid sequence. Below the sequences, the triangle indicates the location of the nucleophilic serine, the rectangle indicates the location of the oxyanion hole motif, and dots mark the nonvariant positions of predominantly hydrophobic residues. The 3 open arrows indicate regions folded as β-strands, and the cylinder indicates the region folded as α-helix in the plant (Solanum cardiophyllum) patatin crystal structure (ProteinDataBank entry 1OXW). The positions of these motifs relative to the nucleophilic serine, which is located in a tight turn, are indicated by the subscript numbers.