The patatin-like lipase family in Gallus gallus

BMC Genomics

Table 3 Primers used for the qPCR analyses.

Gene	Forward/Reverse	Sequence (5'-3')	Melting temperature (°C); Temperature of fluorescence measurement (°C)
PNPLA2	Forward	CAGCACCTTTATCCCTGTCTAC	60; 83
PNPLA2	Reverse	AATTGGATGCTTGTATTGGTG	54
PNPLA3	Forward	GTTGTCCAGGCATTGATCTG	57; 82
PNPLA3	Reverse	ATCACACTCCCCAGCAAAAG	57
PNPLA4	Forward	ATGCTGGAATGAAGCCAGTC	57; 79
PNPLA4	Reverse	CTGGTTTGGTGGAAACATAGC	58
PNPLA6	Forward	CACCGTTGAGTGGCTCAACATG	62; 91
PNPLA6	Reverse	CTCCTCCATCGCCTTGATCAC	62
PNPLA7	Forward	TGTTCCAGAAGCCACCAGAC	59; 86
PNPLA7	Reverse	CTGCGTTCTTCTGCGTACAG	59
PNPLA8	Forward	ACTGTGGCAGGCCATTAGAG	59; 84
PNPLA8	Reverse	TTTCATATCGGCCAGTACCC	57
PNPLA9	Forward	AGGACATGGTTCGGTCTCTG	59; 88
PNPLA9	Reverse	GCGTCATCTCTGCCTTCTTC	59
CGI-58	Forward	GATTCCTGGCTGCTGCTTAC	59; 82
CGI-58	Reverse	CGCTGAACAAGGCTTAGACC	59
β-actin	Forward	AGCTATGAACTCCCTGATGG	57; 84
β-actin	Reverse	ATCTCCTTCTGCATCCTGTC	57
ALAS	Forward	GGCAGCTCAGATGAACCACAAG	62; 82
ALAS	Reverse	CGGGTCTTTGCTTCAGCATAATATC	61
PBGD	Forward	AACTGTGGGAAAACGCTCAG	57; 82
PBGD	Reverse	GGCTCAGGAGCTGACCTATG	61

ISSN: 1471-2164