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Table 1 Study factors collected with control animal microarray data

From: Sources of variation in baseline gene expression levels from toxicogenomics study control animals across multiple laboratories

Category

Study Factor Query

Study

Organism name

 

Animal strain

 

Sex

 

Study number

 

Institution

Subject characteristics

Individual animal identifier

 

Animal supplier

 

Animal age at sacrifice

 

Total body weight at sacrifice

 

Time of day at sacrifice (AM or PM)

Husbandry

Diet name and source

 

Diet availability

 

Number of animal in a cage

 

Number of hours of light per day

 

Was food withheld prior to sacrifice?

 

Fasted > 12 hr?

 

Blood collection during in life study?

 

If yes, time of collection relative to sacrifice

Specimen

Organ Sampled

 

Organ section sampled

 

Organ preservation method

 

Organ weight at sacrifice

Euthanasia

Sacrifice method

 

Exsanguinated?

 

Anesthetic used

Treatment

Vehicle

 

Route of vehicle administration

 

Frequency of dosing

 

Duration of dosing

 

Dose volume

 

Time between dosing and sacrifice

Microarray experiment

RNA extraction protocol

 

Quality parameters for RNA

 

Sample preparation protocol

 

Rounds of amplification

 

Amount of RNA on array

 

Array type used

 

Scanner model