Table 2 Primers sequences of a random selection of 41 EST-STMS.
SSR name | EST Accession number | SSR Type | Forward Primer Sequence (5' to 3') | Reverse Primer Sequence (5' to 3') | Expected Product size (b) | Observed product size (b) | SSR localization |
|---|---|---|---|---|---|---|---|
5 | DY262823 | (AG)11 | AAGGCATAGCAAAGAAGCCA | CTTGGGCCATCATCTACTGG | 203 | 204/236 | cds |
10 | DY263095 | (TC)9 | TCAAAGTTGATTTTCATTTGCC | GGGAACATCATAGTCGGTGC | 165 | 178/180 | 5' UTR |
16 | DY264179 | (TC)13 | ACCTGAGCCCTTTTTGGTTT | GCCAGATCAAGGCTCAAATC | 136 | 133/136 | nd. |
20 | DY264355 | (AT)7 | AAAAACACCTGTGGGACAGC | TAAACACTCCAGGCACCCTC | 123 | 125 | 5' UTR |
21 | DY264533 | (TC)8 | TGATCAGCAACCAATAACCG | AGTCCGTCGTTTGTGATGTG | 240 | 240 | nd. |
25 | DY264633 | (TC)6 | CGGTCAGGTCCTCACATACA | TGATCTTCTTCGCCTCCATT | 206 | 350 | 5' UTR |
26 | DY265129 | (TC)6 | GTTCTCCCCTTCCCTCTCTG | CCAATGATGAAAGCCAAACA | 268 | 300 | nd. |
34 | DY265633 | (TA)6 | TTATGCTCCGGCTGCTTAGT | AAAAGCCACTCGTTACACGG | 165 | 166 | nd. |
43 | DY266190 | (TA)6 | CGAACCACTCCCCATCTCT | TGATGGTGGTGTTCTCCTTG | 130 | 330 | nd. |
115 | DY274953 | (TA)6 | CCCCCTCTTCTTTCACACAA | GGTGAGCAGCCATCTTCTTC | 136 | 136/140 | 5' UTR |
159 | DY280390 | (GA)10 | TTTTTGGCTTTCTGGGTTTG | GCTCCACTGGGATAGCTGAG | 243 | multibands | 5' UTR |
282 | DY294129 | (CT)6 | GGACCAGAAGCAGGTTTTGT | AAAGAGCGATGACCCAAAAA | 201 | 187/201 | cds |
295 | DY294759 | (TC)6 | CACCTTCTCAGGCAATCTCC | TTGAGCGATGTGAAGAGGTG | 133 | 134 | cds |
482 | DY296883 | (GA)10 | CCCCCTCTTTTTCTCTTCCA | TTCTGGGCTGGTAGGTTCAG | 215 | 210/214 | cds |
652 | DY262841 | (GA)11 | TCTTCTGCTGGAAACAAGCC | TGGAAGAGAAGAAACGGTGG | 221 | multibands | 5' UTR |
817 | DY287851 | (TA)17 | CCCAGCTTCCAGAGAAGAGA | GTCAAGAATCAAGCAGGCGT | 195 | 195/219 | 5' UTR |
830 | DY284947 | (TC)6 | TTCATGGCAGCTTGAGTTTC | TTGGTTTCTTTTGGGGATCA | 197 | 197/199 | cds |
1527 | DY292105 | (TC)6 | GCGCGATCACTCTCTTTCTT | ATCGGGTTTGGATTAGGGAC | 114 | 114/116 | cds |
32 | DY265504 | (CAG)6 | CAGATCCTATTGCAGAGGCA | GCCCATTTGTATTGCCATTT | 175 | 178 | 5' UTR |
67 | DY268562 | (AGC)5 | ATGTGGCTCCCTCTTCTCCT | GTGCATAACTGGGCCGTACT | 175 | 192/195 | 5' UTR |
92 | DY272212 | (ATC)5 | CGCAGCTTTTGCATGTTTTA | TGCTGCTAACCCACAGACAG | 242 | 253 | cds |
93 | DY272212 | (CTT)5 | TGCATTTTCACCTCAGCAAC | GGGAGAGAGAGAAAGCCAGC | 212 | 210 | cds |
116 | DY274953 | (AGA)7 | GAATTGGGAGGACGAACTGA | CGAGCCCTAGACAGAGATGG | 252 | 249/252 | cds |
117 | DY275245 | (TCA)6 | AACAAACCCAGAACACTGCC | TGAGTGTGGGCGTAGATTGA | 108 | 108 | 5' UTR |
121 | DY275927 | (TAA)9 | TCCCTATCATCGGCAACTTC | CAATAATGTTAGGCTGGATGGA | 181 | 180 | 3'UTR |
137 | DY277386 | (CAA)5 | CGTCTTGCTCGCTGTATCTG | TCGCTTTTGGGATTTGAGAC | 166 | 166 | 5' UTR |
154 | DY279967 | (GCC)5 | AAGCCTCAAGTCAAGGCAAA | GCCCCATTTTGTATGGAGTG | 107 | 105/108 | 5' UTR |
164 | DY281040 | (GCC)5 | GTTTTCAGCTGGATTCGAGG | CACGTGTCCTCCTGGAACTT | 180 | 181/187 | cds |
175 | DY281748 | (CAG)6N38(GCA)6 | ACAGCAACCCCAGTCACTCT | CGCTCCTCGATTTGAAGAAG | 252 | 252 | 5' UTR |
179 | DY282259 | (CTT)5 | TTCTCTCTCTCGAGCTTCGC | CCCAATCATCCTCCGTTAGA | 210 | 220 | cds |
196 | DY283426 | (CGC)5 | TCTTCTTCCCTGCTTTTCCA | ATCAAGGAGATCCATGTGGG | 274 | 275 | cds |
203 | DY284275 | (CTT)5 | CTTCACAACCAAGGCCATTT | CTGTGTGCGAGCGTATCACT | 205 | 205 | cds |
228 | DY286984 | (GAG)5 | TGAAGGTGCTAGGATTGGCT | CGGACACTCAAAAGCTGACA | 238 | 508 | 5' UTR |
238 | DY288340 | (TTC)5 | CATGTTTCATTGCAAATGCC | TCTGGACATTCCATCACCAA | 272 | 370 | 5' UTR |
338 | DY299973 | (CTT)11 | TTTCTAAAATTTCCTTCATGGC | CAGGTGAAATCTCATCGCCT | 204 | 204/216 | 5' UTR |
418 | DY274485 | (AAT)5 | AAAACAAACGCCACCTAAATG | CAGCAGCTGAAAACACCTGA | 134 | 135 | 3'UTR |
430 | DY275609 | (AAT)7N15(AGC)7 | CCGATACAGCACAAAGCAAA | TGGAAAGAGAGAAGCCAAGC | 129 | 130 | cds |
432 | DY275609 | (GAG)5 | GAGCTCAAAACAATAGCCGC | CATACCTCCCCGTCCATCTA | 226 | 330 | cds |
818 | DY287851 | (TCT)6 | GTAGATTCGTTCAAGGCCCA | GTGAAGCTGGAAGAGATGGC | 134 | 135 | 5' UTR |
1210 | DY295001 | (ATC)5 | GCCAAAATGCATGTTCAAGA | GTGCCAATGATGATCACGTC | 175 | 183 | 5' UTR |
1388 | DY289396 | (GGA)6 | AAAACAAAGCACCCAGATCG | ACGGCAGCAACGAGATAAGT | 138 | 139 | cds |