Evidence of VEEV infection in mouse brain. Mouse brains at various time points after VEEV infection were analyzed by H&E and IHC staining. VEEV appeared in brain at 48 hr post infection (indicated by arrows), which was accompanied by the initiation of inflammation in brain as evident by vessel thickening and endothelial cuffing. At 72 hr post infection VEEV antigen can be detected throughout the mid-brain. Inflammation was evidenced by increased endothelial cuffing and neutrophil infiltration (indicated by arrows). Inflammation was extensive throughout the brain along with increased VEEV antigen in brain at 96 hr post infection.