Figure 11

Simplified model of regulation of differential gene expression in phagocytosis and growth on bacteria. The model depicts some differentially regulated genes in phagocytosis and growth on bacteria, with emphasis on genes encoding putative membrane receptors, signal transducers, transcription factors and selected metabolic pathways. Over- or under-expressed genes are indicated in yellow and grey, respectively. Genes detected in the comparison V12M2 vs. AX2 growing on bacteria are indicated in italics. Phagocytosis leads to overexpression of putative membrane receptors, tetraspanins and metabotropic receptors. Receptor clustering due to bacterial binding, and to the scaffold activity of tetraspanin, may generate signalling complexes containing also GPCR's. Activation of the heterotrimeric G protein [5], putatively via autocrine signals or bacterial metabolites binding to GPCR's, will regulate actin reshaping in the phagocytic cup, favouring phagocytosis. We further suggest that the same signal complex at the membrane of the phagocytic cup or the engulfed phagosome may generate signals regulating gene expression, which can be mediated by a TIR-domain containing protein acting on transcription factors. Up-regulation of genes involved in sterol metabolism leads to increased production of sterols, which we suggest to be preferentially incorporated in phagocytic cups and phagosomal membrane. GABA may accumulate as catabolic product of glutamate, due to the high activity of GadA and GadB glutamate decarboxylase. Whether it is released and acts as autocrine signal is open. Regulation of phosphoinositides may regulate phagosomal maturation and fusion with lysosomes. See Additional Files 1, 2, 3 for DDB ID numbers of genes encoding the protein products indicated. The DDB ID numbers in the lower box, without protein product indication, identify genes, which are strongly stimulated by phagocytosis and encode hypothetical membrane proteins with no identifiable domains.