Identification and characterisation of new splice variants of Jmjd6. (A) Two additional putative exons (green boxes) were identified in addition to the transcript annotated in Ensembl (variant 1) by screening all public EST databases. Alternative splicing using these two exons results in the generation of two additional transcripts of Jmjd6 (variants 2 and 3, respectively). Half arrows and orange lines in the schematic presentation of the transcripts highlight the combination of exons detected by RT-PCR. Numbering of Jmjd6 exons includes the additional identified exon variants 4b and 5. (B) Expression analysis of alternative Jmjd6 transcripts in adult mouse organs and embryonic stages (E) using RT-PCR confirmed the presence of all three transcripts at different mRNA expression levels in the analysed samples. Half arrows in (A) indicate the position of the PCR primers used for the amplification. Blue numbers and arrows in (B) point to PCR fragments amplified from splice variants 1 to 3. Amplification of the housekeeping β-actin gene was used as a loading control. (C) The effects of the different splicing events on the C-termini are shown in the gapped alignment from amino acid 300 onwards. The full length human JMJD6 and murine Jmjd6 proteins are represented in the upper part of the figure. Nearly all novel splice variants identified in the mouse (Mm 2) and in humans (Hs 2–3) are truncated at the C-terminus in comparison to splice variant 1 (Mm 1 and Hs 1) and are predicted to contain no poly-serine stretch ([Ser]n). In contrast, the JmjC domain is not affected (green box). Predicted AT-hook domain, sumoylation recognition site (Sumo), and nuclear export signals (NES) are annotated as depicted at the bottom.