Multiple sequence alignment of homologous Jmjd6 proteins. The sequences of 54 homologous Jmjd6 proteins were used for the calculation of a multiple sequence alignment (ClustalW). The numbering of the residues is according to the mouse protein sequence. Identical residues were shaded in blue and similar residues in magenta. Green shading indicates the predicted catalytic residues of the HxDxnH facial. Sequence insertions in individual species outside of the conserved core regions of the mouse Jmjd6 protein were masked out and the maximum numbers of amino acids in these insertions are indicated [n]. Based on the sequence conservation, a frequency corrected sequence logo was calculated (shown above the alignment) and the mouse sequence conservation was highlighted in colour dots according to their degree of conservation (shown as colour code in percent identity below the alignment). The JmjC domain (light blue bar) and additional identified secondary structure elements (red cylinders: α-helices; yellow and green arrows: β-strands) were annotated to the sequence as indicated at the bottom and the β-strands forming the jelly-roll of the JmjC domain were numbered (I-VIII). Green arrows represent the β-sheet that extends the major sheet outside of the jelly-roll.