Table 1 Primers for real-time quantitative PCR
From: Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley
Chr. | Gene | Forward (5' – 3') | Reverse (5' – 3') | Amplicon (bp) |
|---|---|---|---|---|
1H | HemA2 | CACGCCATCTGTTTGAGGTATC | TCTTTCCCAGGCCTCCACTAT | 120 |
1H | Hor3 | TGTGTTGGCAAACTGCACTTG | TTGTGAGGCCCTTAAGTCGG | 123 |
1H | Hva1 | CTCCACAAGCAGTCGATCCA | GGCCATCTTCGTCTCACGAT | 111 |
1H | Nbs | CAACCTACACCGGAAAACTCATCT | TTGTGAGGCCCTTAAGTCGG | 137 |
2H | Cor14b | CCCAAACAGGTCACCCAAAG | TGCGTGCGAGACTGTCGAC | 139 |
3H | Dhn10 | GGGTTCTCGATCTCTTCTTGCAT | TCTTCCTCCGTCCACCCA | 177 |
4H | Dhn6 | GGACGTACGGCGCTACTGAG | TGGTTCCTCGAGTCTTTATTCTTCA | 150 |
5H | Dhn9 | GTTCCACGTGATCTTCATTCAATAA | TCAGCAAGAAGACACAAGAACACA | 137 |
6H | Dhn8 | CCGTCCTTCTTTCTTGCTTGTG | TATAGCCGCTGTCAAGTAGACCG | 100 |
7H | Ss1 | CTTGGCCGGGATGCGTTA | GGTGTGGGTATCCGAGGGAG | 101 |