Functional genomics reveals increases in cholesterol biosynthetic genes and highly unsaturated fatty acid biosynthesis after dietary substitution of fish oil with vegetable oils in Atlantic salmon (Salmo salar)

Table 2 Lipid class composition of liver of Atlantic salmon (Salmo salar) fed various dietary oils

Lipid class	FO	LO	RO	SO
Total lipid	4.2 ± 0.2	4.8 ± 0.5	4.4 ± 0.5	4.8 ± 0.8
Phosphatidylcholine	17.7 ± 4.0^ab	13.3 ± 0.5^b	19.0 ± 1.6^a	14.5 ± 0.8^ab
Phosphatidylethanolamine	10.1 ± 1.5^a	7.7 ± 1.2^b	9.4 ± 0.7^ab	7.8 ± 0.6^b
Phosphatidylserine	2.6 ± 1.3	3.1 ± 0.5	3.7 ± 0.4	3.1 ± 0.5
Phosphatidylinositol	3.2 ± 1.4	2.8 ± 0.8	4.0 ± 0.3	3.4 ± 0.5
PG/CL	3.2 ± 0.9	2.2 ± 1.1	2.6 ± 0.3	1.8 ± 0.4
Sphingomyelin	1.8 ± 0.3	1.4 ± 0.3	1.8 ± 0.2	1.4 ± 0.3
Lyso-PC	2.8 ± 0.2	2.7 ± 0.4	2.2 ± 0.5	1.9 ± 0.4
Unknown polar lipid	2.0 ± 0.3	2.5 ± 0.2	3.2 ± 0.2	2.8 ± 0.3
Total polar	43.4 ± 6.9^ab	35.7 ± 0.6^b	45.8 ± 3.9^a	36.8 ± 3.2^ab
Total neutral	56.6 ± 6.9^ab	64.3 ± 0.6^a	54.2 ± 3.9^b	63.2 ± 3.2^ab
Cholesterol	15.9 ± 0.9	15.7 ± 1.0	16.0 ± 0.8	15.3 ± 0.5
Triacylglycerol	24.8 ± 7.8	29.4 ± 2.2	23.1 ± 3.5	28.3 ± 2.4
Free fatty acid	7.7 ± 2.3	12.1 ± 1.8	8.0 ± 4.0	10.4 ± 0.8
Steryl ester	6.9 ± 2.1	5.6 ± 2.2	3.8 ± 1.3	5.6 ± 1.9
Unknown neutral lipid	1.3 ± 0.9^b	1.4 ± 0.8^b	3.3 ± 0.5^a	3.6 ± 0.6^a

Values are means ± SD of 4 samples each of tissue pooled from 3 fish. Significance of differences between means were determined by one-way ANOVA followed, where appropriate, by Tukeys multiple comparison post hoc test. Values within a row with a different superscript letter are significantly different (P ≤ 0.05). CL, cardiolipin; Lyso-PC, lysophosphatidylcholine; PG, phosphatidylglycerol. All values are expressed as percentage total lipid, except total lipid which is expressed as percentage of wet weight.

ISSN: 1471-2164