Functional genomics reveals increases in cholesterol biosynthetic genes and highly unsaturated fatty acid biosynthesis after dietary substitution of fish oil with vegetable oils in Atlantic salmon (Salmo salar)

Table 6 Gene expression measured by QPCR in the liver of Atlantic salmon fed vegetable oils in comparison to fish oil.

	LO		SO		RO
	Expression	P value	Expression	P value	Expression	P value
Genes changed on array
isopentenyl-diphosphate Δisomerase	3.263	0.007	3.755	0.008	3.313	0.023
fatty acyl Δ6 desaturase	2.809	0.009	3.215	0.025	3.994	0.004
apolipoprotein B-100	1.535	0.029	1.137	0.624	1.416	0.036
PPARγ	1.053	0.805	1.118	0.649	1.122	0.555
Genes not on array (BLAST to ESTs)
Mevalonate kinase	2.158	0.003	2.334	0.027	2.949	0.008
SREBP2	2.563	0.018	2.095	0.057	2.578	0.011
adipophilin	2.836	0.032	3.469	0.032	1.338	0.459
acyl-CoA oxidase	1.432	0.034	1.227	0.343	1.501	0.043
Reference or no change on array
β-actin	0.773	0.318	0.908	0.694	0.794	0.291
EF1α	1.199	0.146	1.115	0.564	1.091	0.456
AJ425111	1.079	0.714	0.988	0.939	1.153	0.338
7-dehydrocholesterol reductase	2.624	0.017	3.319	0.02	3.826	0.009
carnitine palmitoyl transferase 1A	1.125	0.411	1.173	0.403	1.099	0.477
catalase	0.919	0.617	0.972	0.892	1.1	0.495
PPARβ1	0.861	0.605	0.932	0.778	0.93	0.78
PPARβ2	0.737	0.228	0.715	0.166	0.756	0.333
HMG-CoA reductase	1.204	0.136	1.132	0.566	1.097	0.516
short chain alcohol dehydrogenase	1.014	0.936	0.806	0.343	1.035	0.865

The identity of the genes tested is indicated. Those genes which were significantly changed (n = 5 fish per treatment, p ≤ 0.05) in fish fed linseed oil (LO), soyabean oil (SO) or rapeseed oil (RO) compared to fish oil are indicated in bold. Expression numbers indicate fold change relative to fish oil.

ISSN: 1471-2164