Table 8 Genes, accession numbers and primers used for quantitative PCR
| Â | Acc No | Forward primer | Reverse Primer |
|---|---|---|---|
Genes from Array | Â | Â | Â |
isopentenyl-diphosphate Δisomerase 1 | CK875291 | ACAGCCCTATGGTTATGTGTCATCTC | CAAGGTGAGGCGAATGTTTGAAC |
7-dehydrocholesterol reductase | DW561983 | CTTCTGGAATGAGGCATGGT | ACAGGTCCTTCTGGTGGTTG |
HMG-CoA reductase | BE518590 | CCTTCAGCCATGAACTGGAT | TCCTGTCCACAGGCAATGTA |
Δ6 desaturase | AY458652 | CCCCAGACGTTTGTGTCAG | CCTGGATTGTTGCTTTGGAT |
apolipoprotein B-100 | EG648437 | GCTGTGGCTTTAATCCTTGC | CCATGGTGTCAGTGATCAGG |
catalase | CK874978 | CAACCCCCAGACTCACCTAA | GAAGGTGTGAGAGCCGTAGC |
carnitine palmitoyl transferase 1A | AM230810 | CCTGTACCGTGGAGACCTGT | CAGCACCTCTTTGAGGAAGG |
short chain alcohol dehydrogenase | CK884238 | TCTGCACACGAGAGGCATAC | GTCAGGGCAGTCACAGCATA |
PPARβ1 | AJ416953 | GACCACCAACCCCAATGGCTCGGAT | CAGCCCATTCTCAGCCTGGCACAAG |
PPARβ2 | AM229303 | CCCCCACCATCTTGGTGGCTCAGAC | TAGACCACTCTCTGCTTGCCACAGG |
PPARγ | AJ416951 | ACCCAGGAACCCAGAGTCAGCGGAC | TTTATAAAGTACTGACCCGCCGTCA |
Genes not on array (BLAST to ESTs) | Â | Â | Â |
Mevalonate kinase | DY708590 | CCCTTAATCAGGGTCCCAAT | GGTGCTGGTTGATGTCAATG |
SREBP2 | DY733476 | GACAGGCACAACACAAGGTG | CAGCAGGGGTAAGGGTAGGT |
adipophilin | BM413877 | GCAGACATGGAAGTCGTTGA | ATGGAAATTTGTGGCTCCAG |
acyl-CoA oxidase | DW555420 | AAAGCCTTCACCACATGGAC | TAGGACACGATGCCACTCAG |
Reference (no change in array ept) | Â | Â | Â |
β-actin | AF012125 | ACATCAAGGAGAAGCTGTGC | GACAACGGAACCTCTCGTTA |
EF1α | AF321836 | CTGCCCCTCCAGGACGTTTACAA | CACCGGGCATAGCCGATTCC |
unknown | AJ425111 | AGCCTATGACCAACCCACTG | TGTTCACAGCTCGTTTACCG |