Differential gene expression of the honey bee Apis mellifera associated with Varroa destructor infection

BMC Genomics

Table 2 Characteristics of the RT-qPCR analysis of differentially expressed genes of Apis mellifera

Product name	Honey bee ID	Forward primer sequences	Reverse primer sequence	Amplicon size (bp)	Amplification efficiency^a(R^b)
Krh1	GB14867	ACTCATCAGTTGTTGGTTCTCCTC	TCGTTTGGCTCTTCAGTCTTGTG	118	2.11 (0.99)
non determined	GB18554	TCACACCGATATTCTCATCAAAGG	CTTGTCATTCTTGTTCTCCGATTG	112	1.94 (0.98)
Hr78	GB18358	TGACGAAGTTTAGTTGCTGCTATG	TGTTGTTCCCTATGATCTCTGTCC	107	1.98 (0.99)
Alh	GB17400	ACTTGTGGTAATGCTGGCTGAC	AACGAACGAAGGAAAGGAATAACG	129	2.08 (0.96)
baz	GB10346	ACCAGGAACAAGCGAGTCAGAAG	ACCAGGAACAAGCGAGTCAGAAG	112	2.02 (0.99)

Honey bee IDs refer to the cDNAs selected from the EST microarray experiment (see text).
Krh1 is the housekeeping gene. Forward and reverse primer sequences and the PCR product length are indicated.
^aAmplification efficiencies were calculated from the slope of standard curves as E = 10 [-1/slope] – 1.100% efficiency corresponds to an amplification efficiency of 1; ^bRegression coefficient of linear standard curve.

ISSN: 1471-2164