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Table 2 Gene Ontology comparison.

From: Performance comparison of two microarray platforms to assess differential gene expression in human monocyte and macrophage cells

GO classes

Reference lists

Affymetrix lists

Illumina lists

RNG-86 lists

 

Affy 18373

Illum 16592

RNG 17550

Best 3800 (n = 3735)

P < 0.001 (n = 1876)

Best 3800 (n = 3756)

Pc < 0.001 (n = 1990)

Best 3800 (n = 3549)

Pc < 0.001 (n = 5198)

Immunity and defense

1154

1124

1123

318 * (P < 10-5) §

189 (P < 10-8)

351 (P < 10-7)

205 (P < 10-7)

326 (P < 10-8)

383 (P < 0.09)

Intracellular signaling cascade

818

779

802

217 (P < 0.05)

118 (P < 0.05)

214 (ns)

118 (ns)

208 (P < 0.05)

272 (ns)

Lipid, fatty acid and steroid metabolism

673

647

655

189 (P < 10-3)

107 (P < 10-3)

196 (P < 0.05)

119 (P < 10-3)

178 (P < 0.05)

223 (ns)

Apoptosis

470

457

458

130 (P < 0.05)

77 (P < 0.05)

127 (ns)

75 (ns)

132 (P < 0.05)

163 (ns)

Carbohydrate metabolism

520

502

496

132 (ns)

69 (ns)

147 (P < 0.05)

75 (ns)

143 (P < 10-3)

190 (P < 0.05)

Protein metabolism and modification

2426

2316

2340

584 (P < 10-3)

283 (ns)

624 (P < 10-4)

342 (P < 10-3)

600 (P < 10-8)

836 (P < 10-6)

Signal transduction

2973

2866

2979

617 (ns)

374 (P < 10-3)

591 (ns)

350 (ns)

565 (ns)

733 (P < 10-6)

  1. GO biological process categories over represented in the lists of differentially expressed genes generated on each platform and according to two different selection criteria (the "Best 3800" probes or a Pc < 0.001). The table is ordered by the adjusted P-value of the test of association between RNG-86 list and GO categories. For each platform, list of DEG was compared to the list of all genes represented on the array. Genes belonging to several GO classes are included several times, once for each GO class that is associated with this gene.
  2. * Number of genes in the Affymetrix list annotated to the GO biological process Immunity and defense.
  3. §Adjusted P-value derived from the binomial statistics testing the significance of the enrichment of the Immunity and defense GO category in Affymetrix list.