Expression profiling of a high-fertility mouse line by microarray analysis and qPCR

BMC Genomics

Table 2 Primers used for cDNA synthesis and qPCR

Gene		Sequenz	Accession nos.	bp
Bcl2	rt	GGTCTGCACCTTTAATCCTAGTAC	BC027249	328
	rev	ACATACAGAGGCCTTGTCTCAGAC
	for	GGAATGACATGTTGCTCACATTTAC
Casp6	rt	TAGCCCTTCCACCACGTCCAAC	NM009811	231
	rev	GCGCTGAGAGACCTTTCTGTTC
	for	ACAGACAAGCTGGACAACGTGAC
Ccnd2	rt	CAGGCTTTGAGACAATCCACATC	NM009829	248
	rev	AATGAAGTCGTGAGGGGTGACTG
	for	CTCTGGCCATGAATTACCTGGAC
Cdh2	rt	CCCACCGCTACTGGAGGAGTTG	NM007664	204
	rev	GCCTCTCGTCTAGCCGTCTGA
	for	GGATGAAACGGCGGGATAAAGAG
Cyp11a1	rt	ATACAGAGATACCACCCTCAAATG	NM019779	252
	rev	TCACGGAGATTTTGAACTTCAAT
	for	GATTCCAGCCAAGACTTTGGTAC
H2-Q7	rt	CAATCAACCCTCAGCTCAAGATG	XM622842	293
	rev	TAGGCTCACAGGGAACATGAGAC
	for	CTGAGCCTCTCACCCTGAGATG
Ifi205	rt	GGTGACATTTCTATTTTGGCATCTC	NM172648	188
	rev	CCTTACAGTTGATGTTGTGCCATTG
	for	CAGAAATGCAAATGCCAGTCCTAAG
Inhbb	rt	ACCAGTGACCTGTCAGTTGTTG	XM148966	272
	rev	TGAGTCGCTCCTGGGCTACTTG
	for	GCACTCTGAATTGCGCCTTCTGA
Nr5a2	rt	CTTGGAGCAGTTCAGAGTATTGTG	NM030676	217
	rev	GTCTTCTGCCTGCTTGCTGATTG
	for	CGATCAGCGGGAGTTTGTATGTC
Pi4k2b	rt	CTGCACTCCACGATCACACATG	NM028744	203
	rev	AGCAGCTCTGTCAAATCCTTTGTC
	for	GGCTAGCATTTCCCTTTAAGCATC
Ptgfr	rt	AGTCCAGCTTCACTCGATGCTTG	NM008966	204
	rev	ACAGGTTCCTAAGGACAGCCTTC
	for	TCATTCAGCTCCTGGCCATAATGT
Raet1a	rt	AAATGTATTAGAGGAGGGAGATAAG	NM009016	218
	rev	CTCCAGTTCCACAGGATCCGATG
	for	GGAAAAGCCAAGATCAACCTCAAG
Rgs5	rt	ATGCAGCCCTTAGACTGCAGAAG	NM009063	242
	rev	GAGGCATCTGAGTGAGTGTGTAAC
	for	GCTTCTAAACAGGATTCATTTCAATC
Runx1	rt	TCGGAGATGGACGGCAGAGTAG	NM009821	246
	rev	GACAGAGGAAGAGGTGATGGATC
	for	ATGAAGAACCAGGTAGCGAGATTC
Stat1	rt	ATGTCGCCAGAGAGAAATTCGTG	NM009283	176
	rev	GGTGGACTTCAGACACAGAAATC
	for	TGCCGAGAACATACCAGAGAATC

bp, lengths of amplicons in base pairs; for, forward primer; rev, reverse primer; rt, primer used for cDNA synthesis

ISSN: 1471-2164