Figure 1From: High accuracy mass spectrometry analysis as a tool to verify and improve gene annotation using Mycobacterium tuberculosis as an exampleMS/MS profile of ion M+H 2019.0094. Tandem mass spectrum of a prevalent ion on a particular time point in the LC gradient and ionized on the LTQ-Orbitrap. The peptide fragments randomly on each amide bond, resulting in carboxy-terminal y ions or amino-terminal b ions. After the fragment masses were submitted to Mascot, the peptide was identified as AAEPSWNGQYLVTLSANAK (inset, with detected y and b ions represented) from protein Rv2253 – conserved hypothetical protein.Back to article page