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Table 1 Summary of sequence coverage of the three minimum tile paths (MTPs) used for BAC end sequencing made from three BAC libraries.

From: Re-annotation of the physical map of Glycine max for polyploid-like regions by BAC end sequence driven whole genome shotgun read assembly

  MTP4E MTP4BH MTP4BH MTP2 BH Totals
Vector pBeloBAC11 pCLD04541 pCLD04541 pCLD04541 na
Insertion site Eco RI Bam HI/Hind III Bam HI/Hind III Bam HI/Hind III na
Ploidy inferred diploid diploid polyploid-like mixed na
Number of clones 3,840 4,608 576 8,064 17,088
Mean insert size (kbp) 175 ± 7 173 ± 7 173 ± 7 140 ± 5 na
Clone coveragea 0.7 0.8 0.2 1.4 3.1
BES good reads 3,324 6,772 924 13,473 25,123
BES coverage (Mbp) 2.9 5.0 0.7 9.9 18.5
Predicted gene-like reads 1,512 3,649 498 7,260 12,919
  1. a To calculate the percentage of the soybean genome covered by the clones (clone coverage) in our Eco RI- (MTPE) and Bam HI or HindIII insert libraries (MTP2BH and MTP4BH), the genome size of soybean was assumed to be 1,130 Mb.