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Table 1 Summary of sequence coverage of the three minimum tile paths (MTPs) used for BAC end sequencing made from three BAC libraries.

From: Re-annotation of the physical map of Glycine max for polyploid-like regions by BAC end sequence driven whole genome shotgun read assembly

 

MTP4E

MTP4BH

MTP4BH

MTP2 BH

Totals

Vector

pBeloBAC11

pCLD04541

pCLD04541

pCLD04541

na

Insertion site

Eco RI

Bam HI/Hind III

Bam HI/Hind III

Bam HI/Hind III

na

Ploidy inferred

diploid

diploid

polyploid-like

mixed

na

Number of clones

3,840

4,608

576

8,064

17,088

Mean insert size (kbp)

175 ± 7

173 ± 7

173 ± 7

140 ± 5

na

Clone coveragea

0.7

0.8

0.2

1.4

3.1

BES good reads

3,324

6,772

924

13,473

25,123

BES coverage (Mbp)

2.9

5.0

0.7

9.9

18.5

Predicted gene-like reads

1,512

3,649

498

7,260

12,919

  1. a To calculate the percentage of the soybean genome covered by the clones (clone coverage) in our Eco RI- (MTPE) and Bam HI or HindIII insert libraries (MTP2BH and MTP4BH), the genome size of soybean was assumed to be 1,130 Mb.