Table 1 PCR Primers used throughout this work
Purpose | Target region | Primer name | Sequence (5' → 3') |
|---|---|---|---|
Determination of the are of deletion | ORF 1 | rgx1startfw | TAAGTTTAGCTAAGGCAGAG |
| Â | Â | rgx1startrv | AAATTAAAGAGGCTAGGCTG |
| Â | ORF 3 | rgx1orf3fw | ACTCGTATGCTTGACTTTCTG |
| Â | Â | rgx1orf3rv | CTATCTTGTTTAACCCAGTCAC |
| Â | ORF 4 | rgx1orf4fw | CTCTTTACTCAATCGCCGAC |
| Â | Â | rgx1orf4rv | CCAACAGCAGATTACGAGAC |
| Â | ORF 5 | rgx1orf5fw | CTTATCCATTTCCGTGTTCC |
| Â | Â | rgx1orf5rv | CTAGAATTCAAAGTCGCCAG |
| Â | ORF 10 | rgx1orf10fw | TATAAGTCTGTTTGGTCCCTG |
| Â | Â | rgx1orf10rv | GTATTACTCACGCTTTACCTG |
| Â | ORF 14 | rgx1orf14fw | TAATAACCCAACCTCTACAC |
| Â | Â | rgx1orf14rv | ACACGAGCAGAATATTAGTC |
| Â | ORF 15 | rgx1orf15fw | GTACTCTAGAGACAGAATGGTGGCGCTATCGTC |
| Â | Â | rgx1orf15rv | GTCAGGATCCAGAGCGGTATCAAGCAGTATCC |
| Â | ORF 16 | rgx1orf16fw | ATGTCTACCTTACTGGATACTG |
| Â | Â | rgx1orf16rv | CCGTCACATATTACAAGTTCTG |
| Â | ORF 20 | rgx1orf20fw | ATCCACCTCATCGTTATTCC |
| Â | Â | rgx1orf20rv | GTGGTTAAGAACAATGGAGC |
| Â | ORF 26 | rgx1orf26fw | GTTGACACCATCTACTGCTG |
| Â | Â | rgx1orf26rv | GCTTATCTACGCCGATTCTG |
| Â | ORF 28 | rgx1orf28fw | GTGTTTAACCATAGCCAGAC |
| Â | Â | rgx1orf28rv | TCTAGGTAAGCCTTCAAGAG |
| Â | ORF 29 | rgx1orf29fw | GAACTCCCTAACTTCATCTCAG |
| Â | Â | rgx1orf29rv | CAACCATCTCACTAGACCAC |
| Â | ORF 31 | rgx1orf31fw | TTCTTGTCAACCCAACAGTC |
| Â | Â | rgx1orf31rv | TTTCTACCACCTTTGAGCAG |
| Â | ORF 34 | rgx1orf34fw | GATACGGTAGATATTCTTCC |
| Â | Â | rgx1orf34rv | GAGAGTACATTCTAACTACC |
Determination of the downstream end of the deletion | +500 | orf31do05kFw | GAGGTACAGCGAATACAC |
| Â | +1000 | orf31do1kFw | CAGATGGTGTTCAAGTTCTC |
| Â | +1500 | orf31do15kFw | CTCTTGCTTCCATCAAATCAG |
| Â | +2000 | orf31do2kFw | CGTCAAGTGTTATGTTGTCC |
| Â | +2500 | orf31do25kFw | CGAGATGAAAGATTCACAGC |
| Â | +3000 | orf31do3kFw | GAGGTATCGTGTTCAATGTC |
Genome Walking | Â | GWqm9414gsp1 | CCTTATCACTACCTTCCACCTCCATCTTATACCC |
| Â | Â | GWqm9414gsp2 | CCTCCATCTTATACCCTCTACCCAATTCCC |
| Â | Â | GWRUTC30gsp1 | TACCGCCATCGCAGACTGTTCCCTTTC |
| Â | Â | GWRUTC30gsp2 | TCACTATGAGACGGCAG |
Cre1 amplification | Â | Cre1fw | TCTCTGGGCTCTCTTGTAACC |
| Â | Â | CreIIr | TGCCACTCCTCCTCATGTCAT |
| Â | Â | creF | GTACTTTGGCCCTCGCTGAG |
| Â | Â | creR | CCAGACTGCATAAGGATTCCC |
| Â | Â | creRUTr | AGCAATCAGGTGCAGATATCAC |