Cross-platform comparison of SYBR® Green real-time PCR with TaqMan PCR, microarrays and other gene expression measurement technologies evaluated in the MicroArray Quality Control (MAQC) study

Table 5 Comparison of fold-change results on the discordant genes between SYBR Green PCR Arrays and other platforms

	RT² Profiler PCR Array	TaqMan	StaRT-PCR	QuantiGene	AFX	ABI	AG1	GEH	ILM
*FURIN*	1.16	-43.98	-1.49	-1.42	-1.21	1.04	-1.38	N/A	-1.12
*RB1*	1.73*	-1.92	-1.59	-3.09	-2.23	-1.30	-4.06	-2.14	-2.14
*ABCD1*	-5.44	-7.11	N/A	-3.83	-1.45	2.21	-8.25	2.15	-2.41
*BAG1*	1.66	-1.06	1.85	-1.97	-2.89	-1.00	1.12	-1.01	1.18
*BAG4*	2.66	1.01	N/A	1.96	1.30	-2.25	-2.25	1.13	1.51
*CDK5R1*	45.53	28.92	N/A	21.55	18.11	-1.09	1.56	2.11	14.26
*IGFBP5*	-1.68	-2.69	-1.98	-2.16	-2.68	4.11	-3.26	-2.42	-2.37
*JAK2*	2.11	1.31	N/A	1.25	1.06	1.11	-1.15	1.99	1.06

Fold-change values for the above platforms other than RT² Profiler PCR Arrays are derived from the data provided by MAQC studies [8, 12]. A positive value for fold change indicates a higher expression while a negative value indicates a lower expression in Sample B when compared with Sample A. "N/A" means the gene was not included in the indicated platform. Discordant fold-change values compared to RT² PCR Arrays are underlined and in bold. *The fold-change value for RB1 gene from the PCR Array using the corrected sequence is -1.75 (see text for details).

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