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Table 1 Validation of DNA microarray results. mRNAs were selected for real time RT-PCR analysis from the list of differentially expressed genes in myo1 Δ with p < 0.01(Additional File 1). Fold-change was estimated by a semi-quantitative method as described in the Methods section.

From: Global mRNA expression analysis in myosin II deficient strains of Saccharomyces cerevisiae reveals an impairment of cell integrity functions

Gene name

Fold Change in Microarray

Fold Change (real time RT-PCR)

Up regulated genes

SLT2

2.1

1.8

ECM4

3.2

3.5

SPI1

8.6

13.0

YHR097C

4.1

3.8

ROM1

3.2

3.5

IRA2

3.7

2.3

Down regulated genes

RPL30

3.1

6.3

RPS31

2.6

7.7