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Table 3 Strains used in this study.

From: Global mRNA expression analysis in myosin II deficient strains of Saccharomyces cerevisiae reveals an impairment of cell integrity functions

Strain Genotype Source
BY4741 MAT a his3delta1 leu2delta0 met15delta0 ura3delta0 ATCC
YJR12 (wild type) MAT α trp1 ura3 leu2-3 his3delta1 ADE+ ARG+ cyhR Lab. strain
YJR13 (myo1Δ) MAT a trp1 ura3 leu2-3 his3delta1 ADE+ ARG cyhRmyo1delta::HIS5+ Lab. strain
MGD353-46D (wild type) MAT α trp1-289 ura3-52 leu2-3, 112 his3delta1 ADE+ ARG cyhR B. Rymond
YJR6 (myo1Δ) MAT α trp1-289 ura3-52 leu2-3, 112 his3delta1 ADE+ ARG cyhRmyo1delta::HIS5+ parental MGD353-46D F. Rivera
YJR13 pRS316-RPL30 (myo1Δ pRS316-RPL30) MAT a trp1 ura3 leu2-3 his3delta1 ADE+ ARG cyhRmyo1delta::HIS5+, pRS316-RPL30 A. Brestcher (plasmid)
YJR13 pRS316-RPS31 (myo1Δ pRS316-RPS31) MAT a trp1 ura3 leu2-3 his3delta1 ADE+ ARG cyhRmyo1delta::HIS5+, pRS316-RPS31 A. Brestcher (plasmid)
YJR13 pRS316-MYO1 (myo1Δ pRS316-MYO1) MAT a trp1 ura3 leu2-3 his3delta1 ADE+ ARG cyhRmyo1delta::HIS5+, pRS316-MYO1 F. Rivera (plasmid)
YJR12 pRS316-RPL30 (wild type pRS316-RPL30) MAT α trp1 ura3 leu2-3 his3delta1 ADE+ ARG+ cyhRpRS316-RPL30 A. Brestcher (plasmid)
YJR12 pRS316-RPS31 (wild type pRS316-RPS31) MAT α trp1 ura3 leu2-3 his3delta1 ADE+ ARG+ cyhRpRS316-RPS31 A. Brestcher (plasmid)
YJF1 (myo1Δslt2Δ pRS316-MYO1) MAT a trp1 ura3 leu2-3 his3delta1 ADE+ ARG cyhRmyo1delta::HIS5+, pRS316-MYO1, slt2delta::KANR This study