Table 3 Strains used in this study.
Strain | Genotype | Source |
|---|---|---|
BY4741 | MAT a his3delta1 leu2delta0 met15delta0 ura3delta0 | ATCC |
YJR12 (wild type) | MAT α trp1 ura3 leu2-3 his3delta1 ADE+ ARG+ cyhR | Lab. strain |
YJR13 (myo1Δ) | MAT a trp1 ura3 leu2-3 his3delta1 ADE+ ARG cyhRmyo1delta::HIS5+ | Lab. strain |
MGD353-46D (wild type) | MAT α trp1-289 ura3-52 leu2-3, 112 his3delta1 ADE+ ARG cyhR | B. Rymond |
YJR6 (myo1Δ) | MAT α trp1-289 ura3-52 leu2-3, 112 his3delta1 ADE+ ARG cyhRmyo1delta::HIS5+ parental MGD353-46D | F. Rivera |
YJR13 pRS316-RPL30 (myo1Δ pRS316-RPL30) | MAT a trp1 ura3 leu2-3 his3delta1 ADE+ ARG cyhRmyo1delta::HIS5+, pRS316-RPL30 | A. Brestcher (plasmid) |
YJR13 pRS316-RPS31 (myo1Δ pRS316-RPS31) | MAT a trp1 ura3 leu2-3 his3delta1 ADE+ ARG cyhRmyo1delta::HIS5+, pRS316-RPS31 | A. Brestcher (plasmid) |
YJR13 pRS316-MYO1 (myo1Δ pRS316-MYO1) | MAT a trp1 ura3 leu2-3 his3delta1 ADE+ ARG cyhRmyo1delta::HIS5+, pRS316-MYO1 | F. Rivera (plasmid) |
YJR12 pRS316-RPL30 (wild type pRS316-RPL30) | MAT α trp1 ura3 leu2-3 his3delta1 ADE+ ARG+ cyhRpRS316-RPL30 | A. Brestcher (plasmid) |
YJR12 pRS316-RPS31 (wild type pRS316-RPS31) | MAT α trp1 ura3 leu2-3 his3delta1 ADE+ ARG+ cyhRpRS316-RPS31 | A. Brestcher (plasmid) |
YJF1 (myo1Δslt2Δ pRS316-MYO1) | MAT a trp1 ura3 leu2-3 his3delta1 ADE+ ARG cyhRmyo1delta::HIS5+, pRS316-MYO1, slt2delta::KANR | This study |