Figure 2

Differentially expressed genes and GO term analysis. A) Lineage-specific gene clustering. Shown is a clustering of 1,606 genes to be more than twofold up- or down-regulated in at least one time point after differentiation induction. Genes were grouped into six fate-specific clusters, three with distinct profiles specific for adipogenesis, three with distinct profiles for osteoblastogenesis, and two fate-independent clusters with common profiles in both differentiation lineages. Relative expression levels (log₂ ratios) for genes at different time points are shown and color coded according to the legend at the top (left) and expression profile (mean ± standard deviation) for each cluster (right). B) Distribution of differentially expressed genes for the three main clusters with adipogenesis-related (ARG), osteoblastogenesis-related (ORG), and differentiation lineage-independent (DIG) profiles. C)-D) Numbers of up-regulated and downregulated genes before and after commitment in both lineages. E) Distribution of significantly enriched GO terms for genes with adipogenic-related profiles (ARG: cluster 1–3) before and after commitment. F) Distribution of significantly enriched GO terms for genes with osteogenic-related profiles (ORG: cluster 4–6) before and after commitment. G) Distribution of significantly enriched GO terms for genes with differentiation-independent profile (DIG: cluster 7+8) before and after commitment.