Differentially expressed genes and GO term analysis. A) Lineage-specific gene clustering. Shown is a clustering of 1,606 genes to be more than twofold up- or down-regulated in at least one time point after differentiation induction. Genes were grouped into six fate-specific clusters, three with distinct profiles specific for adipogenesis, three with distinct profiles for osteoblastogenesis, and two fate-independent clusters with common profiles in both differentiation lineages. Relative expression levels (log2 ratios) for genes at different time points are shown and color coded according to the legend at the top (left) and expression profile (mean ± standard deviation) for each cluster (right). B) Distribution of differentially expressed genes for the three main clusters with adipogenesis-related (ARG), osteoblastogenesis-related (ORG), and differentiation lineage-independent (DIG) profiles. C)-D) Numbers of up-regulated and downregulated genes before and after commitment in both lineages. E) Distribution of significantly enriched GO terms for genes with adipogenic-related profiles (ARG: cluster 1–3) before and after commitment. F) Distribution of significantly enriched GO terms for genes with osteogenic-related profiles (ORG: cluster 4–6) before and after commitment. G) Distribution of significantly enriched GO terms for genes with differentiation-independent profile (DIG: cluster 7+8) before and after commitment.