Figure 4

Network of TFs regulating the genes up-regulated with increasing dilution rate. Nodes with thicker outlines contain the TFs found in our analysis (YAP5, YAP6, SMP1, GAT3 and BAS1 do not map into this network). The connectivity among nodes is based on the interactions stored at BioGRID database [58] and the interaction types can be divided in two groups: (1) genetic interactions, which can be detected by dosage rescue, synthetic rescue, synthetic growth defect, synthetic lethality, phenotypic enhancement and phenotypic suppression (blue edges); and (2) protein interaction, detected by affinity capture-MS, affinity capture-western, reconstituted complex and two-hybrid (green edges). See Additional file 3 for details about these interactions. Moreover, nodes are colored according to the expression levels of the genes encoding the TFs and a grey-red scale is used (red color indicates higher expression levels). In this network, the TF expression values from experiments at 0.2 h^-1 are depicted. No significant differences in TF expression values were observed at different dilution rates (see Additional file 1). The network was drawn by using Cytoscape [59].