Table 1 Oligonucleotide primers used in quantitative PCR and quantitative RT-PCR. Amplicon sizes for all genes are 81 bp.
Gene | EST accession number | Primer sequence (5' to 3') |
|---|---|---|
actin | EE597333 | TGGAGATGGTGTTTCCCACAC |
| Â | Â | CCAGCCAAGTCCAAACGAAG |
serpin | EE604017 | GCTCGACCATGGACTGGTTC |
| Â | Â | CTAGATTTCGCCGCGGTAGT |
phenoloxidase | EE598742 | GGCGAGGAGAAGGACTGTGA |
| Â | Â | AATAAGGCAGACCCCATCGG |
tetraspanin-3 | EE600355 | GCATCGGTCAGATCGTGTTG |
| Â | Â | CTTTCAAGGAGCCGAAGCAT |
Adh-classIII | EE599295 | GGTTATGTCTGCTCCTGCCG |
| Â | Â | CCCAAAAGTTCAGCAGCCTC |
TCP1-delta | EE595595 | CGGCTTCAAATAGTTCAGGTGA |
| Â | Â | TTCGAATATCCTTTGGCTTGCT |
GroEL | EE599508 | GTTGTAGCTGGAGGAGGTACTGACC |
| Â | Â | TGTTTGGTCTTCGTTGTTGCC |
citrate synthase gltA | DQ077708 | AAAGGTTGCTCATCATGCGTT |
| Â | Â | GCCATAGGATGCGAAGAGCT |
knottin | DQ308607 | CTGTTCCAAGCCAAAACCGA |
| Â | Â | GATCATGAAGGCGGCCACTA |