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Table 2 Quantitative real-time RT-PCR analysis of selected transcripts.

From: Gene expression patterns of sulfur starvation in Synechocystis sp. PCC 6803

A
Gene 1 hr 3 hr 6 hr
  rRNA trpA lysC rRNA trpA lysC rRNA trpA lysC
cpcA -1.95 ± 0.23 -2.79 ± 0.01 -2.35 ± 0.01 -1.66 ± 0.11 -2.28 ± 0.17 -2.23 ± 0.06 -1.40 ± 0.21 -1.38 ± 0.02 -1.95 ± 0.04
psbA2 -3.08 ± 0.10 -2.97 ± 0.02 -2.52 ± 0.02 -2.03 ± 0.04 -2.07 ± 0.17 -2.01 ± 0.05 -0.94 ± 0.17 -0.45 ± 0.02 -1.02 ± 0.04
nblA2 0.12 ± 0.10 1.20 ± 0.20 1.14 ± 0.15 3.77 ± 0.03 -0.88 ± 0.09 0.25 ± 0.23 2.98 ± 0.09 n.d. 1.99 ± 0.21
cysW 0.80 ± 0.12 n.d. n.d. 6.06 ± 0.10 1.41 ± 0.13 2.54 ± 0.25 4.56 ± 0.38 n.d. 3.57 ± 0.43
B
Gene 1 hr 3 hr 6 hr
hoxH 0.65 ± 0.06 0.83 ± 0.07 0.06 ± 0.05
hoxE 0.52 ± 0.03 0.72 ± 0.05 -0.14 ± 0.06
hypA1 0.22 ± 0.01 0.13 ± 0.06 -0.21 ± 0.07
hypB1 -0.01 ± 0.09 -0.31 ± 0.07 -0.86 ± 0.06
lexA 0.52 ± 0.08 -0.4 ± 0.09 -0.98 ± 0.08
  1. A. Transcript abundances of significantly induced nblA2(ssl0453), cysW(slr1454) and repressed cpcA(sll1578), psbA2(slr1311) genes, relative to stable RNA, trpA, or lysC, as endogenous references. B. Measurements of transcript abundances of hydrogenase-related genes using lysC as an endogenous reference. Mid-exponential cells were harvested and fixed in phenol-ethanol stop solution before sulfate withdrawal (time 0) and 1, 3, 6 hr after sulfate withdrawal. Total RNA was isolated and reverse transcribed. The relative abundances of each transcript were measured by SYBR Green real-time PCR and analyzed according to the comparative C t method. The data are presented as transcript abundances, normalized to a reference mRNA, in a time-point sample relative to control (time 0) on the log2 basis. Errors are the pooled s.e.m. (n = 3). "n.d." – not determined.