Figure 2
From: Deciphering downstream gene targets of PI3K/mTOR/p70S6K pathway in breast cancer
Protein-level validation of p70S6K suppression after RPS6KB1 siRNA treatments in breast cancer cell lines. BT-474 and MCF-7 cells were treated with three siRNAs against RPS6KB1 and one scramble oligo for 72 hours and the protein expressions were detected by Western immunoblotting. The p70S6K expression was significantly downregulated in siRNA-transfected samples when compared to the scramble oligo transfected samples. Beta-actin was used as a loading control.