Figure 5
From: Deciphering downstream gene targets of PI3K/mTOR/p70S6K pathway in breast cancer
Effects of rapamycin, Ly294002 and RPS6KB1 siRNA treatments on apoptosis of breast cancer cell lines. A) The cells were treated with 100 nM rapamycin and 50 μM Ly294002 for 48 hours and the cell morphology was evaluated under the light microscope. B) Caspase-3 activity assay of rapamycin- and Ly294002-treated breast cancer cell lines. C) Caspase-3 activity assay of RPS6KB1 siRNA-treated breast cancer cell lines. The values are averages from two or three independent experiments. Ly294002 induced apoptosis in BT-474 and MDA-361 breast cancer cells, whereas rapamycin had no effect. Similarly, knock-down of RPS6KB1 did not have any effect on apoptosis in BT-474 and MCF-7 breast cancer cells.