Analysis of expressed sequence tags from Actinidia: applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening

Table 3 Functional classification of ESTs and NRs from Actinidia.

Bin Code	Bin Name	% Actinidia ESTs^a	% Actinidia NR sequences^b	% Arabidopsis sequences	% Potato NRs
1	PS	2.18	1.22	0.73	0.75
2	major CHO metabolism	0.86	0.72	0.37	0.54
3	minor CHO metabolism	1.02	0.75	0.45	0.41
4	glycolysis	1.03	0.60	0.23	0.27
5	fermentation	0.38	0.21	0.05	0.07
6	Gluconeogenesis/glyoxylate cycle	0.14	0.11	0.04	0.02
7	OPP	0.25	0.22	0.11	0.12
8	TCA/org. transformation	0.64	0.55	0.27	0.27
9	mitochondrial electron transport/ATP synthesis	0.82	0.71	0.49	0.34
10	cell wall	3.20	2.27	1.90	1.11
11	lipid metabolism	1.78	1.91	1.49	1.32
12	N-metabolism	0.08	0.16	0.09	0.08
13	amino acid metabolism	2.64	2.00	1.07	1.18
14	S-assimilation	0.10	0.08	0.05	0.05
15	metal handling	2.53	0.59	0.34	0.20
16	secondary metabolism	3.02	2.09	1.64	1.42
17	hormone metabolism	2.16	1.99	2.27	1.69
18	co-factor and vitamin metabolism	0.10	0.14	0.16	0.15
19	tetrapyrrole synthesis	0.28	0.26	0.17	0.16
20	stress	4.08	3.33	3.48	2.64
21	redox. regulation	1.02	1.00	0.70	0.64
22	polyamine metabolism	0.29	0.12	0.05	0.07
23	nucleotide metabolism	0.59	0.68	0.57	0.49
24	biodegradation of xenobiotics	0.04	0.08	0.09	0.06
25	C1-metabolism	0.19	0.19	0.14	0.12
26	miscellaneous	4.72	4.59	5.38	3.69
27	RNA	7.86	9.86	10.88	6.82
28	DNA	1.60	2.05	1.99	1.25
29	protein	18.61	16.94	13.31	9.59
30	signalling	3.42	4.77	4.46	3.10
31	cell	3.23	3.04	2.57	1.68
33	development	1.29	1.58	2.24	1.33
34	transport	3.62	3.99	3.49	2.58
35	not assigned	26.23	31.21	38.72	55.79

^a Actinidia NRs were assigned to a Mapman (Thimm et al., 2004) bin according to their nearest Arabidopsis BLAST hit. ESTs belonging to the Actinidia NR were then assigned to the same bin.
^b Potato and Arabidopsis functional assignments were as described [27, 100]

ISSN: 1471-2164