Table 8 Specifications for the new intron mutants used in our study.
mutant | segment location | original sequence | substitution/insertion |
|---|---|---|---|
rps17b-L1 | 258–268 (11 nt) | UGAAGAGAGGU | augagacaacu |
rps17b-L2 | 138–157 (20 nt) | GAUUAGAAAACUCCAUUACU | cuuaaguuaguaaauaccuc |
rps17b-L3 | 22–47 (26 nt) | UGAAGCCGGAUAUGAUGGACUGGGC | uuaAGCCGcuacuacuUGGACUGucg |
rps17b-L4 | 167–189 (23 nt) | AGAAGAGCGCUCAAUGAAGUAGU | uggcuuggguuaguaggugccuc |
rps17b-S1 | 217–231 (15 nt) | AAUUGCUUUCGAAUG | uuucauguguucagc |
rps17b-S2 | 280–286 (7 nt) | UAAGUUG | uacguac |
rps17b-S3 | 246–253 (8 nt) | AUCCAAUG | uagCggcu |
rps17b-S4 | 244–253 (10 nt) | UUAUCCAAUG | cuucaucaac |
rps6b-L1 | 21–54 (34 nt) | CCUUAGAAUUCUAAUGAAUCAGCACGCGCUAACC | guauuuugggugugucccuguuauaaauaauacc |
rps6b-S1 | 19–29 (11 nt) | AUCCUUAGAAU | uuuguuaguaa |
rps6b-S2 | 87–113 (27 nt) | CACAAAUUAGUGCACUAUAAUAAAAAU | uuauaaauagugauaccauuugguaaa |
rps6b-S3 | 21–57 (37 nt) | CCUUAGAAUUCUAAUGAAUCAGCACGCGCUAACCGGC | aaauuccaacguuucccugcaacaugccuuucuuccg |
rps6b-S4 | 38–55 (18 nt) | AUCAGCACGCGCUAACCG | auucccaacagacugucc |
rps6b-S5 | 337–345 | GUAUUAUUU | GgUguucAUUAUUacaU |
ape2-L1 | 159–175 (17 nt) | UGUUACCCUCAUAUUCU | ggguacaauuaauagag |
ape2-L2 | 237–252 (16 nt) | GCAAUAGCUUAGGUAA | ccuucguacuuuuggg |
ape2-S1 | 23–37 (15 nt) | CAAAGAAACAAGGAA | agggcagaaauagaa |
ape2-S2 | 43–57 (15 nt) | AUACAUAAUAUAAAU | aacugguagguacgu |
ape2-S3 | 237–252 (16 nt) | GCAAUAGCUUAGGUAA | caaugaaugagaacuc |
ape2-S4 | 159–175 (17 nt) | UGUUACCCUCAUAUUCU | aaauauuaccuaagcua |
ape2-S5 | 300–322 (23 nt) | CUCGUUACCGACCUUUGAGUUCU | uuaagcuuuuguguuugagaaca |