Table 1 Summary of gene targets evaluated by quantitative real-time PCR.
From: Effects of increased milking frequency on gene expression in the bovine mammary gland
Gene | Amplicon size (bp) | Sense primer (5'→ 3') | Antisense primer (5'→ 3') |
|---|---|---|---|
Microarray-identified genes | |||
ALDH5A1 | 116 | ccttctctgcaggttgaagc | tcccacaaaatacagcatgaa |
CIDEA | 192 | tcctacgacatccactgcac | cccctaccctctcttgatcc |
CTGF | 175 | tgtgcagttggagcaacagt | cttccaggtggaaaaaccaa |
ECM1 | 217 | cgggatatcttgacccttga | agttacgtcgggaaccacac |
FGFR2 | 168 | ttggcgaatcttcatcacag | gtgagatcctgccagaggag |
IGFBP6 | 161 | aaggagagtaagccccaagc | agcacggagtccagatgttt |
MIA | 172 | tgtttctcggtgtcgtcttg | atggtcaagaaacggcagtc |
PHLDA1 | 131 | gcaagtggactctggaccat | tacaatgatgcggaaggaca |
PTN | 215 | gctgaccaagtccaaacctc | tcattttgtttctgcctattgtg |
SERPINF1 | 245 | gcctcagaaagtgacccaga | cgcgatgttccacttgagta |
SPADH1 | 176 | ctgagcacccagcttctttc | acaggctgagagcaggtgat |
Sage-identified genes | |||
GCHFR | 123 | tggccttctctgaaacctgt | tttgacagaacagggccttc |
GLYCAM1 | 115 | caggcaaccacagagtcaga | tgcatcactgggagtgtgtt |
LTF | 107 | tcggttattctggtgccttc | gtccctgtcagccttctctg |
LALBA | 125 | ctctgctcctggtaggcatc | acagacccattcaggcaaac |